Abstract: The disclosure belongs to the technical field of pesticides, and relates to an aminocarboxylic acid chelating agent with antifungal activity and synergistic use thereof with other sterilants. The aminocarboxylic acid chelating agent and salts thereof used alone or in combination can effectively inhibit growth and development of Fusarium, Botrytis cinerea, Pyricularia, Trichoderma, Penicillium and oomycetes with an effective antimicrobial concentration of 0.003-300 mM. They can effectively control wheat scab, rust disease, powdery mildew, rice blast, sheath blight, vegetable gray mold, sclerotinia and other fungal diseases. Combination with organic sulfur, antibiotic, benzimidazole, triazole, methoxyacrylate, succinate dehydrogenase inhibitor sterilants and oomcide drugs, can reduce amount of pesticides applied and improve control effects.

Abstract: The invention provides a ?-tubulin segment of a ?-tubulin gene, and also provides an application of the segment in controlling plant diseases and/or enhancing plant disease resistance, inhibiting pathogenic fungal development and pathogenicity, and improving the drug sensitivity of pathogens to tubulin binding agents. The invention also provides an in-vitro interference preparation including ?TubdsRNA segments of the above-mentioned ?-tubulin gene, and an application of the in-vitro interference preparation in breeding a transgenic plant resistant variety. The RNA interference technology of the ?-tubulin gene of the present invention has green and safe advantages of increasing the drug sensitivity of pathogenic fungi, reducing the level of drug resistance, interfering with pathogenicity, enhancing plant disease resistance, controlling a variety of plant diseases, improving the drug sensitivity to a chemical agent (carbendazim), and prolonging the dsRNAs drug retention period by the carbendazim.

Abstract: The present invention discloses RNAi constructs derived from a Fusarium chitin synthase gene Chs3b, which has a nucleotide sequence as shown by SEQ ID NO: 1. Five distinct RNAi vectors are constructed for 5 different segments of the Chs3b gene (named Chs3b-1, 2, 3, 4, and 5, respectively), and separately transformed into Fusarium. It is found that siRiNAs in the transformed Fusarium shows a significant inhibition in fungal growth, development, and pathogenicity. The expression of the RNAi vectors against Chs3b in plants may inhibit the infection of Fusarium and improve the resistance of the transgenic plants to Fusarium head blight.

Abstract: The present invention discloses RNAi constructs derived from a Fusarium chitin synthase gene Chs3b, which has a nucleotide sequence as shown by SEQ ID NO: 1. Five distinct RNAi vectors are constructed for 5 different segments of the Chs3b gene (named Chs3b-1, 2, 3, 4, and 5, respectively), and separately transformed into Fusarium. It is found that siRiNAs in the transformed Fusarium shows a significant inhibition in fungal growth, development, and pathogenicity. The expression of the RNAi vectors against Chs3b in plants may inhibit the infection of Fusarium and improve the resistance of the transgenic plants to Fusarium head blight.