Abstract: Provided are a cell sorting chip, device, and method based on dielectrophoresis induced deterministic lateral displacement, the chip including a microfluidic channel (4), the microfluidic channel comprising a sample inlet (4.1), a straight channel, and two sample outlets; three groups of electrode array pairs are integrated at a bottom of the straight channel, the three electrode array pairs respectively being a focusing electrode group (1), a sorting electrode group (2), and a separating electrode group (3); the focusing electrode group is used for cell focusing and signal detection; the sorting electrode group is used for single cell sorting; and the separating electrode group is used for single cell separation. High-speed, precise, and lossless target cell sorting can thus be implemented.