Patents by Inventor Xiyu Jia
Xiyu Jia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210371910Abstract: Reagents and methods for analysis of DNA hydroxymethylation are provided. Methods comprise modification of hydroxymethylated cytosine residues with a bulky moiety to protect hydroxymethylated positions from cleavage with a DNA endonuclease. For example, methods may comprise contacting DNA with a glucosyltransferase to glucosylate hydroxymethylated DNA positions and digesting the DNA with a DNA endonuclease to cleave DNA in positions lacking hydroxymethylation. Reagents and kits for hydroxymethylated DNA analysis are also provided.Type: ApplicationFiled: February 8, 2021Publication date: December 2, 2021Inventors: James YEN, Xiyu JIA
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Patent number: 11072817Abstract: In a first aspect, the invention concerns a method for detecting or quantifying DNA methylation at a locus. In one embodiment, a methylation-sensitive endonuclease is formulated together with a polymerase enzyme in an appropriate reaction mixture such that amplification of DNA occurs in a methylation specific manor. Quantitative DNA amplification at selected loci can be used to determine the level of methylation. Kits and reagents for performing such methods are also provided.Type: GrantFiled: August 22, 2017Date of Patent: July 27, 2021Assignee: ZYMO RESEARCH CORPORATIONInventors: Xiyu Jia, Onyinyechi Chimaokereke, Lam Nguyen
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Publication number: 20210163921Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: ApplicationFiled: July 20, 2020Publication date: June 3, 2021Inventors: Stanislav FORMAN, Xiyu JIA
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Patent number: 10717976Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: GrantFiled: December 7, 2015Date of Patent: July 21, 2020Assignee: ZYMO RESEARCH CORPORATIONInventors: Stanislav Forman, Xiyu Jia
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Patent number: 10464961Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.Type: GrantFiled: April 28, 2015Date of Patent: November 5, 2019Assignee: ZYMO RESEARCH CORPORATIONInventors: Stanislav Forman, Xiyu Jia
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Publication number: 20180135113Abstract: In a first aspect, the invention concerns a method for detecting or quantifying DNA methylation at a locus. In one embodiment, a methylation-sensitive endonuclease is formulated together with a polymerase enzyme in an appropriate reaction mixture such that amplification of DNA occurs in a methylation specific manor. Quantitative DNA amplification at selected loci can be used to determine the level of methylation. Kits and reagents for performing such methods are also provided.Type: ApplicationFiled: August 22, 2017Publication date: May 17, 2018Inventors: Xiyu Jia, Onyinyechi Chimaokereke, Lam Nguyen
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Publication number: 20170204397Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: ApplicationFiled: December 7, 2015Publication date: July 20, 2017Inventors: Stanislav FORMAN, Xiyu JIA
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Publication number: 20160319271Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: ApplicationFiled: December 7, 2015Publication date: November 3, 2016Inventors: Stanislav FORMAN, Xiyu JIA
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Publication number: 20160222373Abstract: A universal column is provided which allows purification methods utilizing centrifugation, syringe coupling and/or use of a vacuum source. Methods for using the universal column and kits comprising the universal column are described.Type: ApplicationFiled: November 6, 2015Publication date: August 4, 2016Inventor: Xiyu JIA
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Publication number: 20160031922Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.Type: ApplicationFiled: April 28, 2015Publication date: February 4, 2016Inventors: Stanislav FORMAN, Xiyu JIA
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Patent number: 9206469Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: GrantFiled: July 17, 2013Date of Patent: December 8, 2015Assignee: ZYMO RESEARCH CORPORATIONInventors: Stanislav Forman, Xiyu Jia
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Patent number: 9051563Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.Type: GrantFiled: January 12, 2012Date of Patent: June 9, 2015Assignee: Zymo Research CorporationInventors: Stanislav Forman, Xiyu Jia
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Publication number: 20140349977Abstract: Methods for determining if a patient has, or is at risk of having, and autism spectrum disorder by detecting epigenetic changes in the genome of the patient. For example, a method can comprise determining the methylation status of one or more genes in a blood sample.Type: ApplicationFiled: October 12, 2012Publication date: November 27, 2014Applicant: ZYMO RESEARCH CORPORATIONInventors: Xueguang Sun, Xiyu Jia
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Publication number: 20140051844Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.Type: ApplicationFiled: July 17, 2013Publication date: February 20, 2014Inventors: Stanislav Forman, Xiyu Jia
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Publication number: 20130065233Abstract: In a first aspect, the invention concerns a method for detecting or quantifying DNA methylation at a locus. In one embodiment, a methylation-sensitive endonuclease is formulated together with a polymerase enzyme in an appropriate reaction mixture such that amplification of DNA occurs in a methylation specific manor. Quantitative DNA amplification at selected loci can be used to determine the level of methylation. Kits and reagents for performing such methods are also provided.Type: ApplicationFiled: March 2, 2011Publication date: March 14, 2013Applicant: ZYMO RESEARCH CORPORATIONInventors: Xiyu Jia, Onyinyechi Chimaokereke, Lam Nguyen
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Publication number: 20120184725Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.Type: ApplicationFiled: January 12, 2012Publication date: July 19, 2012Inventors: Stanislav Forman, Xiyu Jia
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Publication number: 20120064521Abstract: Reagents and methods for analysis of DNA hydroxymethylation are provided. Methods comprise modification of hydroxymethylated cytosine residues with a bulky moiety to protect hydroxymethylated positions from cleavage with a DNA endonuclease. For example, methods may comprise contacting DNA with a glucosyltransferase to glucosylate hydroxymethylated DNA positions and digesting the DNA with a DNA endonuclease to cleave DNA in positions lacking hydroxymethylation. Reagents and kits for hydroxymethylated DNA analysis are also provided.Type: ApplicationFiled: December 8, 2010Publication date: March 15, 2012Inventors: James Yen, Xiyu Jia
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Patent number: 7892811Abstract: The present invention relates to the simple, gentle, and efficient extraction of biological material from Escherichia coli (E. coli). The use of E. coli in research laboratories depends on the ability to prepare lysates to isolate the desired products under investigation. The present invention includes methods and engineered E. coli strains that are capable of rapid controlled lysis or herein “autolysis”. The XJa strains were made from JM109 and the XJb strains from BL21 by insertion of the ? R or (? SR) lytic endolysin gene to replace the tightly regulated araB gene. Thus, arabinose becomes a non-metabolizable inducer and the controlled autolysis phenotype is induced by the PBAD promoter by the presence of saturating arabinose. Upon induction of the bacteriophage ?R endolysin, the E. coli remains intact but is efficiently lysed after one freeze-thaw cycle. The present invention is usable with many different buffer systems and is flexible in this regard.Type: GrantFiled: August 16, 2005Date of Patent: February 22, 2011Assignee: Zymo Research CorporationInventors: Xiyu Jia, Jan Kostal, Jonathan Anthony Claypool
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Patent number: 7867751Abstract: The invention provides apparatus, reagents, and methods for rapidly isolating plasmid DNA from a bacterial alkaline lysate.Type: GrantFiled: November 21, 2006Date of Patent: January 11, 2011Assignee: Zymo Research CorporationInventor: Xiyu Jia
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Patent number: 7858363Abstract: Apparatus, reagents, and methods for isolating plasmid DNA from bacteria by alkaline lysis using a solid or immobilized P2 and/or P3 reagent in combination with a DNA-binding matrix.Type: GrantFiled: July 22, 2006Date of Patent: December 28, 2010Assignee: Zymo Research CorporationInventor: Xiyu Jia