Abstract: A method and system for sensing or characterizing a biomarker, such as a proteolytic enzyme or nucleic acid. The system comprises a nanopore sensor to determine a current modulation of a sample including a biomarker, and a predetermined substrate or nucleic acid probe current modulation signature for comparison to a current signature from the nanopore sensor. The nanopore sensor includes a nanopore membrane between two fluid compartments, and a power supply in electrical contact with the membrane to provide an electric potential difference between the fluid compartments. A detector is used to detect an electrical current through the nanopore as the polypeptide substrate, or components thereof, transits the nanopore under an applied electric potential difference between the first and second fluid compartments.

Abstract: A method and system for sensing or characterizing a biomarker, such as a proteolytic enzyme or nucleic acid. The system comprises a nanopore sensor to determine a current modulation of a sample including a biomarker, and a predetermined substrate or nucleic acid probe current modulation signature for comparison to a current signature from the nanopore sensor. The nanopore sensor includes a nanopore membrane between two fluid compartments, and a power supply in electrical contact with the membrane to provide an electric potential difference between the fluid compartments. A detector is used to detect an electrical current through the nanopore as the polypeptide substrate, or components thereof, transits the nanopore under an applied electric potential difference between the first and second fluid compartments.

Abstract: The present invention is a nanopore stochastic sensing system comprising modified protein pores for detection and sequencing of oligonucleotides. The system comprises a genetically modified protein pore with a variety of non-covalent bonding recognition sites to significantly slow down the translocation of ssDNA in the pores. The present invention also describes identification and application of DNA fingerprints, which are a sequence of small current modulation events for the determination of the sequence of ssDNA molecules. In separate embodiments the present invention describes a system and a method for the detection of monovalent cations, liquid explosives, water-insoluble compounds, biomolecules and oligonucleotides. The system comprising a wild-type or genetically modified protein pore with or without a molecular adaptor. Analyte samples and mixtures are added along with specially synthesized ionic liquids.

Abstract: The present invention is an array nanopore stochastic sensing system for detection of single biomolecules and oligonucleotides. The system comprises a multi-channel system with multiple genetically modified protein pores for detection of analytes using the pattern recognition mechanism. By monitoring current blockade patterns, identity of single biomolecules can be determined in complex mixtures.