Patents by Inventor Xu Hua FU

Xu Hua FU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210163940
    Abstract: The disclosure describes that both Cas9 and Cpf1 enzymes can exhibit potent nickase activities on an extensive class of mismatched dsDNA targets. These properties allow the production of efficient nickases for a chosen dsDNA target sequence, without modification of the nuclease protein, using guide RNAs with a variety of patterns of mismatch to the intended DNA target. In parallel to the nicking activities observed with purified Cas9 in vitro, sequence-dependent nicking for both perfectly matched and partially mismatched target sequences in a Saccharomyces cerevisae in vivo system was observed.
    Type: Application
    Filed: October 29, 2020
    Publication date: June 3, 2021
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Xu Hua FU, Andrew FIRE, Justin D. SMITH