Abstract: The present invention is to provide multilayered multiple quantum dot-doped nanoparticles, each of the multiple quantum dot-doped nanoparticles has a structure consisting of an inorganic core particle, a quantum dot-embedded layer, and a silica/quantum dot composite shell. The multiple quantum dot-doped nanoparticles can be used to detect biomolecules with improved quantum yield (QY) and brightness while maintaining a large area covered by the quantum dots and stable bonds of the quantum dots. Therefore, the multiple quantum dot-doped nanoparticles are suitable for bioapplications, including bioplatforms and highly sensitive methods for detecting biomolecules.

Abstract: The present invention provides a biological use including a bioplatform and a method for detecting a high-sensitivity biomolecule by providing improved quantum yield (QY) and brightness when detecting biomolecules by preparing nanoparticles, which are doped in multiple quantum dots and have a structure of inorganic core particles, a quantum dot buried layer and a silica/quantum dot composite shell, while maintaining a high occupancy and stable bonding.