Abstract: An ultra-tough physical hydrogel based on hierarchical energy associative dissipation network is provided, which the field of polymeric materials. The stock solution of a hydrogen donor is prepared by dissolving the hydrogen donor in deionized water; the stock solution is mixed with a hydrogen acceptor, deionized water and a 10 wt % ammonium persulfate (APS) solution; the mixture is cured in a closed container; after reactants with specific functional groups and appropriate spatial structures are mixed, a hierarchical heterogeneous structure with a dynamic recovery capability can be spontaneously formed in polymerization reaction, and the mechanical properties of the hydrogel are kept stable through several energy associative dissipation paths.

Abstract: An electrically controlled depolarizer based on a crossed-slit waveguide (3) includes a horizontal-slit waveguide (1), a 45-degree polarization rotation waveguide (2), a pair of modulation electrodes (4) and the crossed-slit waveguide (3).

Abstract: Provided are an AAV capsid protein mutant and an encoding nucleic acid, and a corresponding vector and a host cell thereof. Also provided are an adeno-associated virus vector containing the AAV capsid protein mutant, a recombinant adeno-associated virus particle constructed therefrom and carrying a gene expression cassette, a preparation method therefor, and the use thereof in treating diseases.

Abstract: The disclosure provides a novel design of guide RNA and uses thereof. In one aspect, the guide RNA comprises a spacer sequence flanked by a direct repeat sequence at both the 5? end and the 3? end of the spacer sequence.

Abstract: The invention provides novel engineered CRISPR/Cas effector enzymes, such as Cas13 (e.g., Cas13e) that substantially maintain guide-sequence-specific endonuclease activity and substantially lack guide-sequence-independent collateral endonuclease activity compared to the corresponding wild-type Cas. Also provided are polynucleotides encoding the same, vectors or host cells comprising the polynucleotides or engineered Cas, and method of use, such as in RNA-based target gene transcript knock down.

Abstract: A method and an apparatus for pushing vehicle information, a user account server, and user equipment are provided. The method includes: receiving an entry event that is of a vehicle and that is sent by a parking management server, and obtaining geo-fence information of a parking lot managed by the parking management server; obtaining, by a user account server, a user account bound to the license plate number of the vehicle; obtaining a location determining result of user equipment corresponding to the user account; and pushing the entry event of the vehicle to the user equipment when the current location of the user equipment is within a geo-fence range.

Abstract: Provided is an AAV mutant that efficiently infects supporting cells. Specifically, provided is a gene expression vector used for treating hearing disorders, and the described expression vector is an AAV mutant. In the AAV mutant, an expression cassette of a therapeutic gene used for treating hearing disorders is inserted or carried, and additionally, the AAV mutant contains a gene sequence that encodes a viral capsid protein mutant.

Abstract: Provided is a method of preparing a bioactive scaffold for inducing tendon regeneration, the method includes decellularizing a fresh tendon tissue and to obtain a decellularized tendon sheet scaffold or slice scaffold, and adding ECM materials to the decellularized tendon sheet scaffold or slice scaffold.