Abstract: This invention provides an automated system and method that allows a user to track the movement of an object, such as at least one cell amongst a plurality of cells as it moves from one point to another over time in order for the user to determine how the at least one cell functions. The user is able to track the movement of the at least one cell and determine if the at least one cell structure has changed, such as the at least one cell experiencing cell division or if two or more cells merge together. This user is able to optimally track the at least one cell to find an approximate location of at least one the cell as it moves in time and distance. Thus, this invention provides the user with a means to track cell movement to study the cell function.

Abstract: A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes defining target identification settings to identify at least two different target sets of image objects, defining target identification settings to identify at least two different target sets of image objects, and defining one or more measurements to be performed using said pair-wise linking relationship(s).

Abstract: A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes providing functions for selecting predetermined image analysis procedures, the functions allowing the user to define: at least one first target identification setting for identifying a first target set of image objects in the image data; at least one second target identification setting for identifying a second target set of image objects in the image data; a relationship between the first and second set of image objects; and one or more measurements which are to be reported for the image data, the measurements being conducted using the defined relationship.

Abstract: This invention, which provides a method for detecting a corruption in an image acquired from a biological sample, includes: providing at least one image of at least one cell; generating the image of the at least one cell over a period of time; determining if the at least one image of the at least one cell is corrupted; applying a wavelet transform, Fourier transform, or other frequency decomposing transform to the at least one image to decompose the at least one image into a plurality of sub-images, wherein the plurality of sub-images have a plurality of low frequency channels, a plurality of middle frequency channels and a plurality of high frequency channels; calculating a ratio based on an energy level of the plurality of low frequency channels and the plurality of middle frequency channels; and removing the at least one image of at least one cell if the at least one image is corrupted.

Abstract: This invention provides an automated system and method that allows a user to track the movement of an object, such as at least one cell amongst a plurality of cells as it moves from one point to another over time in order for the user to determine how the at least one cell functions. The user is able to track the movement of the at least one cell and determine if the at least one cell structure has changed, such as the at least one cell experiencing cell division or if two or more cells merge together. This user is able to optimally track the at least one cell to find an approximate location of at least one the cell as it moves in time and distance. Thus, this invention provides the user with a means to track cell movement to study the cell function.

Abstract: A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes providing functions for selecting predetermined image analysis procedures, the functions allowing the user to define: at least one first target identification setting for identifying a first target set of image objects in the image data; at least one second target identification setting for identifying a second target set of image objects in the image data; a relationship between the first and second set of image objects; and one or more measurements which are to be reported for the image data, the measurements being conducted using the defined relationship.

Abstract: A method of forming a buried contact junction without forming a buried contact trench and without a disconnection gap in the current path by using a tapered polysilicon profile and a large angle tilt buried contact implant is described. A layer of gate silicon oxide is provided over the surface of a semiconductor substrate. A first polysilicon layer is deposited overlying the gate silicon oxide layer. The first polysilicon layer is etched away where it is not covered by a buried contact mask to provide an opening to the semiconductor substrate wherein the first polysilicon layer is tapered such that the bottom of the opening has a width the size of the planned buried contact and wherein the top of the opening has a width larger than the size of the planned buried contact. Ions are implanted at a tilt angle into the substrate within the opening whereby the ions penetrate the substrate laterally underlying with said first polysilicon layer to form the buried contact.