Abstract: Provided herein are engineered Cas13 proteins with enhanced collateral activity. The engineered proteins comprise RNA binding domains (RBDs) fusions within an active site-proximal loop within a higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain. Also provided are compositions comprising and methods of using the engineered Cas13 proteins to detect target nucleic acids at attomolar sensitivity.

Abstract: The present disclosure provides a single nucleotide polymorphism (SNP) molecular marker combination for genetic relationship identification of a Huaxi cattle, use, and an identification method, and belongs to the technical field of genotyping. The present disclosure provides a SNP molecular marker combination for genetic relationship identification of a Huaxi cattle, where the genetic relationship identification of the Huaxi cattle is completed with at least 70 of 1,000 sites. In the present disclosure, the 1,000 sites are the SNP molecular markers distributed on 29 autosomes, and two adjacent SNP molecular markers on a same autosome have an average distance of 2 Mb. A minimum allele frequency (MAF), an expected heterozygosity (HExp), and a polymorphism information content (PIC) of the SNP molecular marker combination have average values of 0.4846, 0.4983, and 0.3741, respectively; and when a maternal genotype is unknown, there is a cumulative probability of exclusion (CPE) of 0.9999999999999.

Abstract: Disclosed herein is a device for detecting nucleic acids, the device comprising a CRISPR-Cas13a-mediated graphene field-effect transistor comprising a source electrode; a drain electrode; a gate electrode; a detection channel; where the channel comprises a CRISPR-Cas13a-mediated graphene layer; where Cas13a is operative to function as an effector protein that targets a specific RNA sequence for cleavage based on a recognition of the RNA sequence by crRNA. Disclosed herein too is a device for detecting nucleic acids, the device comprising a CRISPR-Cas12a-mediated graphene field-effect transistor comprising a source electrode; a drain electrode; a gate electrode; a detection channel; where the channel comprises a CRISPR-Cas12a-mediated graphene layer; where Cas12a is operative to function as an effector protein that targets a specific DNA sequence for cleavage based on a recognition of the DNA sequence by crRNA.

Abstract: The present disclosure discloses a camera lens group including, sequentially from an object side to an image side along an optical axis, a stop; a first lens having positive refractive power; a second lens having negative refractive power; a third lens having positive refractive power; a fourth lens having refractive power; a fifth lens having refractive power; a sixth lens having positive refractive power; and a seventh lens having negative refractive power. An aperture number Fno of the camera lens group satisfies: Fno?1.53. A distance TTL along the optical axis from an object-side surface of the first lens to an imaging plane of the camera lens group and half of a diagonal length ImgH of an effective pixel area on the imaging plane of the camera lens group satisfy: TTL/ImgH?1.31.

Abstract: The present disclosure discloses an optical imaging lens assembly which includes sequentially from an object side to an image side along an optical axis, a first lens, a second lens, a third lens, a fourth lens, a fifth lens and a sixth lens. The first lens has a positive refractive power with a convex object-side surface; the second lens has a refractive power with a concave image-side surface; the third lens has a refractive power with a convex object-side surface; the fourth lens has a refractive power with a concave object-side surface; the fifth lens has a refractive power; and the sixth lens has a refractive power with a concave object-side surface. A maximum effective radius DT11 of the object-side surface of the first lens and a maximum effective radius DT61 of the object-side surface of the sixth lens satisfy 0.6<DT11/DT61<1.

Abstract: The present disclosure provides a genomic selection (GS) breeding chip of Huaxi cattle and use thereof, and relates to the technical field of molecular breeding. The present disclosure provides a molecular marker combination for whole-genome genotyping of Huaxi cattle, where a genotyping object involves 112,177 single nucleotide polymorphisms (SNPs) and 3 Indels, including seven marker panels in total. In the present disclosure, a genome-wide breeding chip constructed with the molecular marker combination has functional relevance, pertinence and effectiveness, innovation, comprehensiveness, practicality, and cost-effectiveness. This breeding chip also shows uniform distribution of chromosomes in the whole-genome, high coverage, and moderate throughput of markers. This breeding chip has desirable compatibility with existing commercial chips and high cost performance.

Abstract: The present disclosure discloses an optical imaging lens assembly including, sequentially from an object side to an image side along an optical axis, a first lens having refractive power; a second lens having refractive power with a convex object-side surface and a concave image-side surface; a third lens having refractive power; a fourth lens having negative refractive power; a fifth lens having negative refractive power with a concave object-side surface and a convex image-side surface; and a sixth lens having refractive power with a concave object-side surface and a convex image-side surface. A distance TTL along the optical axis from an object-side surface of the first lens to an imaging plane of the optical imaging lens assembly and a total effective focal length f of the optical imaging lens assembly satisfy: TTL/f<1.

Abstract: The present disclosure discloses an optical imaging system including, sequentially from an object side to an image side along an optical axis, a first lens having positive refractive power; a second lens having refractive power; a third lens having negative refractive power; a fourth lens having refractive power with a convex object-side surface and a concave image-side surface; a fifth lens having refractive power; and a sixth lens having negative refractive power. Half of a maximal field-of-view Semi-FOV of the optical imaging system satisfies Semi-FOV<15°. A distance TTL along the optical axis from an object-side surface of the first lens to an imaging plane of the optical imaging system, half of a diagonal length ImgH of an effective pixel area on the imaging plane and an effective focal length f of the optical imaging system satisfy 1.9 mm<TTL*ImgH/f<2.7 mm.

Abstract: The present disclosure discloses an optical imaging lens group including, sequentially from an object side to an image side along an optical axis, a first lens, a second lens, a third lens, a fourth lens, a fifth lens and a sixth lens. The first lens has a refractive power, an object-side surface of the first lens is a concave surface, and an image-side surface of the first lens is a convex surface; the second lens has a positive refractive power; the third lens has a negative refractive power; the fourth lens has a refractive power; the fifth lens has a negative refractive power, and the sixth lens has a refractive power. An effective focal length f2 of the second lens and a radius of curvature R3 of an object-side surface of the second lens satisfy 1.5<f2/R3<2.

Abstract: Disclosed are an optical detection method and an optical detection apparatus for a fatigue state of a user, and an optical detection device, this application relates to the field of detection technology, and is for improving accuracy of detection of the fatigue state. An optical detection method includes: irradiating eyes of the user with an infrared light; obtaining an intensity of an infrared light reflected by the eyes of the user; and determining whether the user is in a fatigue state based on the intensity of the reflected infrared light.

Abstract: The present application discloses an array substrate, a display apparatus and a method of fabricating an array substrate. The array substrate has a plurality of first bottom-gate type thin film transistors each of which including a metal oxide active layer and a plurality of second bottom-gate type thin film transistors each of which including a silicon active layer.

Abstract: A combined sedimentation tank including a flocculation tank, a transition zone and a sedimentation tank which are sequentially connected along a direction that water flows. The sedimentation tank includes a sloping plate zone and a horizontal flow zone. The length of the sloping plate zone is a quarter of the total length of the sedimentation tank. A return pipe is arranged at the bottom of the sloping plate zone. Some of sludge in the sloping plate zone is returned to the flocculation tank through the return pipe to increase the concentration of the suspended solids in the flocculation tank, thereby forming the sediments.

Abstract: The present disclosure discloses a camera lens group including, sequentially from an object side to an image side along an optical axis, a stop; a first lens having positive refractive power; a second lens having negative refractive power; a third lens having positive refractive power; a fourth lens having refractive power; a fifth lens having refractive power; a sixth lens having positive refractive power; and a seventh lens having negative refractive power. An aperture number Fno of the camera lens group satisfies: Fno?1.53. A distance TTL along the optical axis from an object-side surface of the first lens to an imaging plane of the camera lens group and half of a diagonal length ImgH of an effective pixel area on the imaging plane of the camera lens group satisfy: TTL/ImgH?1.31.

Abstract: A power semiconductor device and a method of fabricating such a power semiconductor device are disclosed. In the method, spacers are formed, which cover sidewalls of a source polysilicon layer and reside on trench portions around the source polysilicon layer. As such, a contact is allowed to be directly formed above the source polysilicon layer, eliminating the need for a special photomask for defining a connection between the contact and the gate electrode, reducing the number of required steps, lowering the process cost and avoiding the risk of contact of the subsequently-formed contact above the source polysilicon layer with a gate polysilicon layer. With the spacers protecting a second oxide layer, during the subsequent formation of a source electrode, the implantation of some n-type ions into the second oxide layer, which may degrade the properties of the second oxide layer, is prevented.

Abstract: A method of querying a status of an electronic insurance policy includes: receiving an inputted policy number; querying an initial status and an operation flow set of the electronic insurance policy corresponding to the policy number when the policy number is a valid policy number; obtaining a mapped status subset according to a status total set to which the initial status belongs and a status that each operation flow in the operation flow set is mapped in the status total set; and displaying the initial status of the electronic insurance policy and the obtained status subset.

Abstract: A method for creating an underwriting decision tree includes: acquiring a sample training set including different sample attributes; calculating an entropy value gain that represents an effect of an attribute on an underwriting result of each attribute, according to the underwriting result of a sample of each attribute in the sample training set; taking the attribute with a highest entropy value gain as a current node of the underwriting decision tree, and dividing a sub-attribute corresponding to the attribute with the highest entropy value gain as a next node of the current node; extracting a divided sample training subset of the sub-attribute from the sample training set; determining the sample training subset as the sample training set, and calculating the entropy value gain of the sub-attribute recursively and dividing the sub-attribute till the divided sub-attribute of the next node satisfies a preset condition of becoming a leaf node of the underwriting decision tree.

Abstract: The present application discloses an array substrate, a display apparatus and a method of fabricating an array substrate. The array substrate has a plurality of first bottom-gate type thin film transistors each of which including a metal oxide active layer and a plurality of second bottom-gate type thin film transistors each of which including a silicon active layer.

Abstract: Provided herein are systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and cytosine deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided.

Abstract: The present disclosure discloses a camera lens assembly. The camera lens assembly includes, sequentially along an optical axis from an object side to an image side, a first lens having a positive refractive power; a second lens having a negative refractive power; a third lens having a refractive power; a fourth lens having a refractive power; a fifth lens having a refractive power; a sixth lens having a refractive power, where an object-side surface of the sixth lens is a convex surface, and an image-side surface of the sixth lens is a convex surface; and a seventh lens having a negative refractive power, where an object-side surface of the seventh lens is a convex surface. An effective focal length f2 of the second lens and a total effective focal length f of the camera lens assembly satisfy: ?3?f2/f<?1.5.

Abstract: The present disclosure discloses an optical imaging lens assembly including, sequentially from an object side to an image side along an optical axis, a first lens having refractive power; a second lens having refractive power with a convex object-side surface and a concave image-side surface; a third lens having refractive power; a fourth lens having negative refractive power; a fifth lens having negative refractive power with a concave object-side surface and a convex image-side surface; and a sixth lens having refractive power with a concave object-side surface and a convex image-side surface. A distance TTL along the optical axis from an object-side surface of the first lens to an imaging plane of the optical imaging lens assembly and a total effective focal length f of the optical imaging lens assembly satisfy: TTL/f<1.