Abstract: Provided herein are HPK-1 inhibitors, such as a compound of Formula X. Also provided are pharmaceutical compositions comprising the HPK-1 inhibitors, methods of using HPK-1 inhibitors, for example, for treating cancer. Method of preparing such HPK-1 inhibitors and novel synthetic intermediates are also provided.

Abstract: Disclosed are an antibody drug conjugate, a preparation method therefor and an application thereof, which are in particular, a conjugate of an anti-PD-L1 antibody and a TLR7 and/or TLR8 agonist, a pharmaceutical composition thereof, a preparation method therefor and an application thereof. In the present invention, a modified anti-PD-L1 antibody having mutated cysteine is obtained by means of gene editing, basically retains the structure of the original antibody, and may be used for the construction of antibody drug conjugates. By means of anti-tumor experiments, it has been discovered that the obtained antibody drug conjugate has good activity, such as strong anti-tumor activity, which may significantly improve the survival rate of tumor-bearing animals, and significantly reduce toxicity.

Abstract: Disclosed are HPK-1 inhibitors having a structure represented by Formula (X), pharmaceutical compositions comprising the HPK-1 inhibitors, methods of using the HPK-1 inhibitors, such as treating cancers, methods of preparing the HPK-1 inhibitors, and the synthetic intermediates.

Abstract: Disclosed in the present invention are a pyridine-2-amine derivative and a pharmaceutical composition and use thereof. The pyridine-2-amine derivative can be used as a TLR8 selective agonist, has the characteristics of high selectivity, strong activity and high safety, can be used for preventing and/or treating diseases related to TLR activity, for example, diseases caused by or related to pathogen infection, immunological diseases, inflammation, and tumors, can also be used for preparing a vaccine adjuvant to enhance immune response, and has better application prospects and research and development value.

Abstract: Provided is a gRNA targeting HPK1 and a method for editing HPK1 gene. The method can knock out the T cell HPK1 gene, enhance the T cell killing activity, increase the Th1 cytokine level of peripheral blood mononuclear cells, and knock out of the T cell HPK1 gene can also down-regulate the expression of PD-1 and TIM3 on the T cell surface and can inhibit the T cell depletion.

Abstract: The present invention discloses a compound of general formula I, or a pharmaceutically acceptable salt, a stereoisomer, an ester, a prodrug, a solvate and a deuterated compound thereof, a composition comprising the compound of general formula I, and use of the compound of general formula I, or the pharmaceutically acceptable salt, the stereoisomer, the ester, the prodrug, the solvate and the deuterated compound thereof for preparing a medicament for treating a disease associated with the serine/threonine kinase family (MAP4Ks), and preferably for preparing a medicament for treating a disease associated with hematopoietic progenitor kinase 1 (HPK1).

Abstract: Provided is a gRNA targeting HPK1 and a method for editing HPK1 gene. The method can knock out the T cell HPK1 gene, enhance the T cell killing activity, increase the Th1 cytokine level of peripheral blood mononuclear cells, and knock out of the T cell HPK1 gene can also down-regulate the expression of PD-1 and TIM3 on the T cell surface and can inhibit the T cell depletion.

Abstract: Disclosed are HPK-1 inhibitors having a structure represented by Formula (X), pharmaceutical compositions comprising the HPK-1 inhibitors, methods of using the HPK-1 inhibitors, such as treating cancers, methods of preparing the HPK-1 inhibitors, and the synthetic intermediates.