Abstract: The present disclosure provides a method for preparing natural starch microspheres with a uniform particle size. The method includes the following steps: heating natural starch and water for gelatinization to form a water phase; controlling a temperature of a substance including a low-polarity solvent to form an oil phase; dispersing the water phase into the oil phase to obtain a mixed solution; treating the mixed solution under reduced pressure, and carrying out separation to obtain preliminarily solidified starch microspheres; and washing the preliminarily solidified starch microspheres to remove the residual oil phase on the surface, and drying to obtain the natural starch microspheres. Compared with the present technology, the method of the present disclosure can retain the molecular structure of the natural starch and has a high yield, and the prepared natural starch microspheres have a uniform particle size and can load water-soluble and fat-soluble loading objects.