Patents by Inventor Xuecheng JIAO
Xuecheng JIAO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240124853Abstract: Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.Type: ApplicationFiled: July 6, 2021Publication date: April 18, 2024Inventors: Hao Hong, Gage James, Yi Xiao, Na Zhang, Xuecheng Jiao, Yulei Ma, Huiyan Mou, Zujian Wang, Kaihua Sun, Xiang Li, Tong Zhao, Shan Cao
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Publication number: 20240117395Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.Type: ApplicationFiled: July 6, 2021Publication date: April 11, 2024Inventors: Hao Hong, Gage James, Yi Xiao, Na Zhang, Xuecheng Jiao, Yulei Ma, Huiyan Mou, Zujian Wang, Kaihua Sun, Ru Jia, Fang Liu, Wenjing Liu
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Publication number: 20240093167Abstract: Provided are an esterase mutant and use thereof. The amino acid sequence of the esterase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include an N51G site.Type: ApplicationFiled: March 2, 2021Publication date: March 21, 2024Inventors: Hao Hong, James Gage, Yi Xiao, Na Zhang, Xuecheng Jiao, Yiming Yang, Xiang Wang, Junqi Zhao
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Publication number: 20240084271Abstract: Provided are an amino acid dehydrogenase mutant and use thereof. The amino acid sequence of the amino acid dehydrogenase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include a K144G site.Type: ApplicationFiled: March 21, 2021Publication date: March 14, 2024Inventors: Hao Hong, James Gage, Yi Xiao, Na Zhang, Xuecheng Jiao, Rui Li, Yanqing Zhang, Yiming Yang
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Publication number: 20240010997Abstract: Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.Type: ApplicationFiled: April 25, 2021Publication date: January 11, 2024Inventors: Hao HONG, Gage JAMES, Na ZHANG, Xuecheng JIAO, Yulei MA, Shan CAO, Yibing CHENG, Kaihua SUN
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Publication number: 20240002890Abstract: Provided are a lipase mutant and use thereof. The amino acid sequence of the lipase mutant has a sequence shown in SEQ ID NO: 1, that is, sites at which amino acid mutations occur include a V154L site.Type: ApplicationFiled: March 2, 2021Publication date: January 4, 2024Inventors: Hao Hong, James Gage, Yi Xiao, Na Zhang, Xuecheng Jiao, Ru Jia, Wenjing Liu, Ye Liu
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Publication number: 20230407352Abstract: The present disclosure relates to synthesis of enantiomerically rich key drug intermediates as a means for manufacturing of thiocarbamate derivatives as A2A adenosine receptor (A2AR) inhibitors. More particularly, the present disclosure provides a viable efficient technology using enzymatic biotransformation process which utilizes cheaper substrate for production of high value key intermediates for A2AR inhibitors.Type: ApplicationFiled: October 29, 2021Publication date: December 21, 2023Applicant: iTeos Belgium SAInventors: Xuecheng Jiao, Michael Deligny, Stefano Crosignani, Xiangjun Jiang
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Publication number: 20230235300Abstract: Provided are a transaminase mutant and an application thereof. Compared with an amino acid sequence shown in SEQ ID NO:1, an amino acid sequence of the transaminase mutant includes at least one of the following mutation sites: L166, K149, K146, A168, H73, F133, H82, E24, V194, T294, A295, G235 and F236. The mutant of the present invention has the improved catalytic activity for a transammonization reaction of ketone substrates, and is suitable for industrial production of chiral amines.Type: ApplicationFiled: May 29, 2020Publication date: July 27, 2023Inventors: Hao HONG, Gage JAMES, Yi XIAO, Na ZHANG, Xuecheng JIAO, Yulei MA, Huiyan MU, Shan CAO
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Publication number: 20230227797Abstract: Provided are a transaminase mutant and use thereof. The transaminase mutant has an amino acid sequence obtained by mutation of an amino acid sequence shown in SEQ ID NO:1, the mutation at least includes one of the following mutation site combinations: T7C+S47C, Q78C+A330C, V137C+G313C, A217C+Y252C and L295C+C328C; or the transaminase mutant has an amino acid sequence which has the mutation sites in the mutated amino acid sequence and has 80% or more identity with the mutated amino acid sequence. The transaminase mutant realizes the change of protein structure and functions, reduces the enzyme amount, increases the enantiomeric excess (ee) value of a product, and reduces the difficulty of post-processing, so that the transaminase mutant may be suitable for industrial production.Type: ApplicationFiled: October 28, 2019Publication date: July 20, 2023Inventors: Hao HONG, Gage JAMES, Yi XIAO, Yulei MA, Na ZHANG, Xuecheng JIAO, Huiyan MU, Yibing CHENG, Shan CAO
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Patent number: 11702640Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.Type: GrantFiled: February 8, 2018Date of Patent: July 18, 2023Assignee: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.Inventors: Hao Hong, James Gage, Jiangping Lu, Xuecheng Jiao, Na Zhang, Rui Li, Kejian Zhang, Yu Zhang
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Patent number: 11603521Abstract: Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.Type: GrantFiled: August 17, 2018Date of Patent: March 14, 2023Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTDInventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Xuecheng Jiao, Rui Li, Kejian Zhang, Yu Zhang, Yiming Yang
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Publication number: 20230002795Abstract: Provided is a ketoreductase mutant and a method for producing chiral alcohol using the same. The ketoreductase mutant has a sequence with amino acid mutations in the sequence shown in SEQ ID NO:1. The mutation sites include at least one of the following positions: 6th position, 21st position, 42nd position, 58th position, 61st position, 76th position, 87th position, 94th position, 96th position, 108th position, 113th position, 117th position, 144th position, 146th position, 147th position, 149th position, 151st position, 152nd, 156th position, 165th position, 177th position, and 198th position.Type: ApplicationFiled: June 13, 2019Publication date: January 5, 2023Inventors: Hao HONG, James GAGE, Yi XIAO, Na ZHANG, Xuecheng JIAO, Kejian ZHANG, Yiming YANG
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Publication number: 20220403421Abstract: Disclosed are a ketoreductase mutant and a method for producing a chiral alcohol. The ketoreductase mutant has an amino acid sequence obtained by the mutation of the amino acid sequence shown in SEQ ID NO: 1, and the mutation includes a mutation siteK200H. In the present disclosure, the mutant obtained by mutation takes a ketone compound as a raw material, the chiral alcohol may be efficiently produced by stereoselective reduction, and the stability is greatly improved, which is suitable for popularization and application to the industrial production of the chiral alcohol.Type: ApplicationFiled: November 7, 2019Publication date: December 22, 2022Inventors: Hao HONG, Gage JAMES, Yi HSIAO, Na ZHANG, Xuecheng JIAO, Kejian ZHANG, Yiming YANG, Xiang WANG, Yanqing ZHANG
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Patent number: 11407982Abstract: Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.Type: GrantFiled: November 6, 2017Date of Patent: August 9, 2022Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTDInventors: Hao Hong, Gage James, Jiangping Lu, Xuecheng Jiao, Na Zhang, Rui Li, Kejian Zhang
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Publication number: 20220002683Abstract: An amino acid sequence of the monooxygenase mutant is obtained by mutation of an amino acid sequence shown in SEQ ID NO: 1, and the mutation at least includes one of the following mutation sites: 45-th site, 95-th site, 106-th site, 108-th site, 114-th site, 186-th site, 190-th site, 191-th site, 249-th site, 257-th site, 393-th site, 436-th site, 499-th site, 500-th site, 501-th site, 503-th site, 504-th site, 559-th site, and 560-th site.Type: ApplicationFiled: November 5, 2018Publication date: January 6, 2022Inventors: Hao HONG, Gage JAMES, Jiangping LU, Xuecheng JIAO, Na ZHANG, Kejian ZHANG, Rui LI, Yu ZHANG, Yiming YANG
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Publication number: 20210292717Abstract: Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.Type: ApplicationFiled: August 17, 2018Publication date: September 23, 2021Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Xuecheng Jiao, Rui Li, Kejian Zhang, Yu Zhang, Yiming Yang
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Publication number: 20210024904Abstract: Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.Type: ApplicationFiled: November 6, 2017Publication date: January 28, 2021Applicant: Asymchem Life Science (Tianjin) Co., LtdInventors: Hao HONG, Gage JAMES, Jiangping LU, Xuecheng JIAO, Na ZHANG, Rui LI, Kejian ZHANG
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Publication number: 20200362318Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.Type: ApplicationFiled: February 8, 2018Publication date: November 19, 2020Inventors: Hao HONG, James GAGE, Jiangping LU, Xuecheng JIAO, Na ZHANG, Rui LI, Kejian ZHANG, Yu ZHANG