Abstract: The disclosure relates to the technical field of sterilizing and disinfecting materials, and specifically relates to an ?-substituted phenyl structure-containing compound, a preparation method thereof, and a disinfectant. The ?-substituted phenyl structure-containing compound according to the disclosure could achieve a bactericidal effect by promoting the coagulation and denaturation of the protein of pathogenic microorganisms. In particular, it has a good killing effect on pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis var. niger spores. Besides, it has no corrosive effect on metals, no irritating odor, good water solubility, and is green and environmentally friendly. Therefore, it could be widely used in various industries as an effective ingredient of a disinfectant.