Abstract: Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-?5) and an application of the pIFN-?5, where the method for preparing pIFN-?5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-?5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-?5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-?5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-?5 gene to obtain the pIFN-?5. The recombinant pIFN-?5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).

Abstract: Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-?5) and an application of the pIFN-?5, where the method for preparing pIFN-?5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-?5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-?5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-?5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-?5 gene to obtain the pIFN-?5. The recombinant pIFN-?5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).

Abstract: A recombinant protein and a vaccine composition for porcine epidemic diarrhea (PED) are provided. The recombinant protein is a fusion protein formed by connecting the truncated segment of S protein (Spike protein) from porcine epidemic diarrhea virus (PEDV) in tandem with the Fc fragment of porcine IgG, and the truncated fragment of S protein is preferably selected from N-terminal domain (NTD) with sialic acid binding activity in S1 subunit of S protein, neutralizing epitope domain (COE) and multiple B-cell epitopes in S2 subunit; the vaccine composition contains recombinant protein and adjuvants. The recombinant protein of the application can produce IgG antibody and neutralizing antibody titers of rather high level after immunizing mice, and the proportions of CD3+CD4+, CD3+CD8+ lymphocytes and the concentrations of IFN-? and IL-4 in lymphocytes are significantly increased.