Abstract: A monoclonal antibody used for specifically binding to CAR molecules, which is characterized in that the monoclonal antibody comprises a light chain and a heavy chain, the light chain comprises CDR regions having an amino acid sequence as shown in SEQ ID NO: 1-3, and the heavy chain comprises CDR regions having an amino acid sequence as shown in SEQ ID NO: 5-7. By using the described monoclonal antibody or a CAR-T cell detection kit, the steps of CAR-T cell detection are simple, detection time is short, and the detection results are stable. An anti-FMC63 ScFv antibody can reduce the cost of detecting universal anti-CD19 CAR-T cells.

Abstract: The present disclosure provides a fully enclosed cell culture system, including at least an incubator, a bioreactor, a gas flow assembly, a liquid flow assembly, a temperature adjustment assembly, and a central controller. The system realizes a thermostatic incubation environment. Cells are incubated by perfusion, and a fully enclosed integrated process from cell activation, infection and amplification to finished product harvesting is achieved. By axially stirring the cells and an incubation liquid in a tank body, a radial shear force is reduced, which effectively protects the cells and improves the cell yield. In the system of the present disclosure, gases are separately introduced, ensuring that the content of each gas component is stable in the incubation process. The cell incubation is not directly affected by changes of outside gases in the cell incubation process, which improves culture efficiency.

Abstract: The present invention provides a glycosylated CEA-specific single-chain antibody, and a chimeric antigen receptor (CAR) targeting a glycosylated CEA, which can be used in manufacture of an agent or a medicament for diagnosis or treatment of a tumor overexpressing glycosylated CEA.

Abstract: The invention provides a full-automatic cell culture system, at least comprising: a control module, a control platform, a dark field microscope for on-line observation of cell culture, a cell incubator shaker and a cell culture bag. The control platform is connected to the dark field microscope for on-line observation of cell culture and the cell incubator shaker respectively, and the control module is connected to the control platform. The invention also provides a culture bag support frame for a continuous cell incubator shaker, the continuous cell incubator shaker, a non-contact sensor connector, the cell culture bag and the dark field microscope for on-line observation of cell culture which are related to the full-automatic cell culture system, thereby realizing continuous culture and observation of cells.

Abstract: The present invention provides a glycosylated CEA-specific single-chain antibody, and a chimeric antigen receptor (CAR) targeting a glycosylated CEA, which can be used in manufacture of an agent or a medicament for diagnosis or treatment of a tumor overexpressing glycosylated CEA.

Abstract: The invention provides a full-automatic cell culture system, at least comprising: a control module, a control platform, a dark field microscope for on-line observation of cell culture, a cell incubator shaker and a cell culture bag. The control platform is connected to the dark field microscope for on-line observation of cell culture and the cell incubator shaker respectively, and the control module is connected to the control platform. The invention also provides a culture bag support frame for a continuous cell incubator shaker, the continuous cell incubator shaker, a non-contact sensor connector, the cell culture bag and the dark field microscope for on-line observation of cell culture which are related to the full-automatic cell culture system, thereby realizing continuous culture and observation of cells.

Abstract: The present invention relates to the field of pharmacochemistry. Disclosed are fluorinated and azido-substituted pyrimidine nucleoside derivatives, particularly compounds of the formula shown below: Also disclosed are methods of preparation and uses for these compounds. The compounds can be used for preparing medicaments for treating diseases such as tumors and viral infections, and can be used separately or in combination with other medicaments. The compounds also have effective activity against diseases such as tumors and viral infections, while having few side effects, and thus have potential application value.

Abstract: The present invention relates to the field of pharmacochemistry. Disclosed are fluorinated and azido-substituted pyrimidine nucleoside derivatives, and preparation methods and uses thereof. The structural formula is as shown (I). These compounds can be used for preparing medicaments for treating diseases such as tumors and viral infections, and can be used separately or in combination with other medicaments. The compounds also have effective activity against diseases such as tumors and viral infections, while having few side effects, and thus have potential application value.