Abstract: A method for detecting a tension force defect of a steel cable includes the following steps: step S10: providing an excitation sensor on a first position of a steel cable to be detected and providing a detection sensor on a second position of the steel cable; step S20: obtaining a value of a tension force on the steel cable and obtaining a first display diagram corresponding to the value of the tension force; step S30: loading a white noise signal on the excitation sensor through a power amplifier; step S40: acquiring a detection signal collected by the detection sensor; step S50: uploading the detection signal to a personal computer (PC) and performing a Fast Fourier Transform (FFT) process on the detection signal; step S60: determining whether there is a defect in the steel cable, and if so, performing step S70; step S70: determining a defect position of the steel cable.

Abstract: A method for detecting a tension force defect of a steel cable includes the following steps: step S10: providing an excitation sensor on a first position of a steel cable to be detected and providing a detection sensor on a second position of the steel cable; step S20: obtaining a value of a tension force on the steel cable and obtaining a first display diagram corresponding to the value of the tension force; step S30: loading a white noise signal on the excitation sensor through a power amplifier; step S40: acquiring a detection signal collected by the detection sensor; step S50: uploading the detection signal to a personal computer (PC) and performing a Fast Fourier Transform (FFT) process on the detection signal; step S60: determining whether there is a defect in the steel cable, and if so, performing step S70; step S70: determining a defect position of the steel cable.

Abstract: This invention relates to a method for preparing site-specific mutations in double-stranded plasmid DNA which comprises the steps of (a) double digesting a plasmid having a site A, which contains a target sequence, and sites B and C, which flank site A, with restriction endonucleases A and B to produce fragment I and with restriction endonucleases A and C to produce fragment II, wherein restriction endonuclease A produces a 3' or 5' overhang; (b) denaturating fragments I and II to form a mixture; (c) reannealing the mixture from step (b) to produce parental homoduplex fragments I and II, heteroduplex fragment III, and heteroduplex fragment IV; (d) extending the 3' ends of the heteroduplex fragments; and (e) ligating the heteroduplex fragments to produce the double-stranded plasmid DNA mutant.

Abstract: A method of making a biologically inactive polypeptide active is disclosed. Activity is imparted to the polypeptide through treatment with an exogenous peptide sequence. The nature of the exogenous peptide sequence is disclosed.