Abstract: A method for preparing an activated expanded lymphocyte stock solution includes co-culturing, in a serum-free medium, a lymphocyte activator with peripheral blood mononuclear cells obtained via autologous peripheral whole blood centrifugal extraction, and thus completing lymphocyte culture activation. The cultured activated lymphocytes are placed into a serum-free medium. Subculturing is performed to prepare lymphocyte activation culture. A serum-free medium having a volume which is 5 to 10 times of the lymphocyte activation culture is added to the lymphocyte activation culture. Expansion culture is performed, wherein the number of expansion culture generations is 1 to 5. A step of centrifuging, washing, and collecting the activated expanded lymphocytes is performed.

Abstract: The present disclosure provides a testis-targeted lycopene (LYC)/ZIF-90 nanocomposite and a preparation method and use thereof, belonging to the technical field of targeted drugs. In the present disclosure, a ZIF-90 metal-organic framework (MOF) material is combined with LYC through aldol condensation; when entering areas of inflammatory response and oxidative stress response, ZIF-90 may disintegrate and collapse in a slightly-acidic environment, releasing the LYC, thereby avoiding easy oxidation of the LYC and improving a bioavailability of the LYC; in addition, the disintegrated and collapsed ZIF-90 may also be utilized by organisms, thus greatly reducing a damage to the organisms. Moreover, the LYC/ZIF-90 nanocomposite is further loaded with follicle-stimulating hormone (FSH).