Abstract: The invention provides an Escherichia coli for synthesizing L-valine, a construction method and use thereof. The Escherichia coli of the invention is designated as Escherichia coli W3110 and was deposited in China Center for Type Culture Collection (Address: Bayi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022. The recombinant Escherichia coli takes Escherichia coli as a starting strain, and a transcription regulation factor is overexpressed to obtain a recombinant Escherichia coli. The recombinant Escherichia coli for synthesizing L-valine of the invention is fermented in a 5 L fermentor with trace dissolved oxygen to test strains, the yield of L-valine reaches 112 g/L, and the OD of the bacterium is 104.

Abstract: A clock data recovery circuit and a clock data recovery method are provided. The clock data recovery circuit includes a time delay loop (100), a frequency locking loop (200) and a deserializer (300). The time delay loop (100) is configured to delay input data according to a phase of a clock signal to realize phase alignment; the frequency locking loop (200) is connected to the time delay loop (100), and is configured to adjust a frequency of the clock signal according to the delayed input data to make the frequency of the clock signal be consistent with a frequency of the input data; and the deserializer (300) is respectively connected to the time delay loop (100) and the frequency locking loop (200), and is configured to deserialize the input data according to the clock signal.

Abstract: A set of primers and probes for simultaneous detection of Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) and a method for detecting CymMV, ORSV, and CymRSV, along with a method for their detection, are disclosed. The method involves designing multiplex real-time quantitative PCR detection primers and probes for CymMV, ORSV, and CymRSV and applying these primers and probes to the real-time quantitative PCR simultaneous detection of CymMV, ORSV, and CymRSV. It allows for faster detection of CymMV, ORSV, and CymRSV, taking only one-third of the time compared to uniplex real-time quantitative PCR technology, thereby reducing testing costs by approximately ? to ½ for each sample. The primers and probes are highly specific and sensitive, with a sensitivity as low as 1 to 10 copies. It provides an efficient and feasible detection method for early detection and prevention of CymMV, ORSV, and CymRSV.

Abstract: Disclosed are a dynamic error measurement apparatus, system, and method for an electricity meter. The measurement apparatus includes a test signal generation unit configured to generate a voltage test signal and two-circuit current test signals, output the voltage test signal to a measurement unit, and output the two-circuit current test signals to the measurement unit and a current summation unit; the measurement unit configured to determine two-circuit electric energy values based on the voltage test signal and the two-circuit current test signals, and output the two-circuit electric energy values to a calculation control unit; the current summation unit configured to determine a combined current signal based on the two-circuit current test signals; and the calculation control unit configured to determine a total electric energy value based on the two-circuit electric energy values. The system includes a standard meter and a measurement apparatus.

Abstract: Health data management methods, a health data management device and a background device are provided. A health data management device can perform unified storage and unified processing for health data from different background devices by using the same data format, and health data of the same health type may have the same health index identifier. Therefore, the health data in different background devices may be interconnected, thereby improving flexibility and efficiency of use of the health data, reducing development costs, and improving development efficiency.