Abstract: The present invention provides an ascorbate peroxidase mutant MaAPX1M36K and an application thereof, belonging to the technical field of biotechnology. An amino acid sequence of the mutant MaAPX1M36K of the present invention is shown in SEQ ID NO. 3. In the present invention, the mutant MaAPX1M36K reconstruction protein is obtained by the method of prokaryotic expression. It is found that the mutant MaAPX1M36K improves catalytic efficiency by nearly 5 times, providing a technical reference for further study and application of APX1.

Abstract: The present invention provides an ascorbate peroxidase mutant MaAPX1M36K and an application thereof, belonging to the technical field of biotechnology. An amino acid sequence of the mutant MaAPX1M36K of the present invention is shown in SEQ ID NO. 3. In the present invention, the mutant MaAPX1M36K reconstruction protein is obtained by the method of prokaryotic expression. It is found that the mutant MaAPX1M36K improves catalytic efficiency by nearly 5 times, providing a technical reference for further study and application of APX1.

Abstract: Fungal trisaccharide ester compounds and agents containing the fungal trisaccharide ester compounds as active ingredients for preventing and controlling plant fungal diseases. A Pezicula sp. strain SC1337 was isolated from endophytes of a branch of bald cypress. The strain SC1337 is capable of producing five compounds exhibiting significant inhibitory activities against twelve common plant pathogenic fungi and preservative effect on fruits. Thus, the strain SC1337 can be used to prepare the five compounds exhibiting significant inhibitory activities against twelve common plant pathogenic fungi and preservative effect on fruits and used for preventing and controlling plant pathogenic fungi and fruit preservation.

Abstract: A citrus preservative includes 0.05%-0.5% of polyhexamethylene guanidine hydrochloride, 0.05%-0.2% of benzimidazole bactericide, 0.02%-0.15% of polyethenoxy and the balance water.

Abstract: The use of polyhexamethylene guanidine hydrochloride as preservative for preventing and controlling citrus sour rot and its application are disclosed by the present invention. The citrus preservative calculated by 100% total mass fraction comprises 0.05%-0.5% polyhexamethylene guanidine hydrochloride, 0.05%-0.2% of one or more selected from the group consisting of a mixture of several of the following: 0.05%-0.2% imidazole fungicidebactericide, benzimidazole fungicidebactericide, pyrimethanil, fludioxonil and azoxystrobin, and 0.02%-0.15% polyethenoxy ether emulsion or quaternary ammonium salt emulsion, and the rest is water. The citrus preservative can significantly inhibit the decay of citrus fruit such as Citrus microcarpa during storage, especially the incidence of sour rot. So the commercial value of citrus is obviously increased.

Abstract: The use of polyhexamethylene guanidine hydrochloride as preservative for preventing and controlling citrus sour rot and its application are disclosed by the present invention. The citrus preservative calculated by 100% total mass fraction comprises 0.05%-0.5% polyhexamethylene guanidine hydrochloride, 0.05%-0.2% of one or more selected from the group consisting of a mixture of several of the following: 0.05%-0.2% imidazole fungicidebactericide, benzimidazole fungicidebactericide, pyrimethanil, fludioxonil and [or] azoxystrobin, and 0.02%-0.15% polyethenoxy ether emulsion or quaternary ammonium salt emulsion, and the rest is water. The citrus preservative can significantly inhibit the decay of citrus fruit such as Citrus microcarpa during storage, especially the incidence of sour rot. So the commercial value of citrus is obviously increased.