Abstract: A microfluidic platform comprising an inlet, a fluidic chamber, one or more trapping arrays, each trapping array comprising pillars separated by gaps, an outlet, and a droplet generator fluidly coupled to the inlet. The droplet generator may accept one or more particles and output particle-laden droplets comprising a particle surrounded by an aqueous solution surrounded by a carrier oil. The particle-laden droplets directed from the droplet generator, through the inlet, to the fluidic chamber, may be immobilized by the one or more trapping arrays. The droplet generator may generate a continuous flow of carrier oil through the inlet and through the fluidic chamber. The continuous flow of carrier oil may induce one or more microvortices at the one or more trapping arrays.

Abstract: A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

Abstract: A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.