Abstract: The present invention relates to application of gossypol and its optical isomers to preparation of an anti-Coronavirus drug. Specifically disclosed is application of pharmaceutically acceptable salts, solvates, isotopes, stereoisomers, stereoisomer mixtures, and tautomers of the gossypol and its optical isomers to preparation of a drug for preventing and/or treating a disease caused by a Coronavirus. The Coronavirus is MERS-CoV, SARS-CoV, or SARS-CoV-2. It is found in the present invention for the first time that the gossypol and its optical isomers can inhibit the activity of Coronavirus 3CL proteases to achieve an anti-Coronavirus effect. The half maximal inhibitory concentrations of gossypol and (?)-gossypol to SARS-CoV proteases and SARS-CoV-2 3CL proteases are all lower than 10 ?M. The anti-Coronavirus 3CL protease effect is good. Therefore, the gossypol and its optical isomers have the potential for use in the preparation of a drug for preventing and/or treating novel Coronavirus infections.

Abstract: The PWM waveform generation device comprises a time-division multiplexing module, wherein the time-division multiplexing module is configured for receiving a first preprocessing signal and a second preprocessing signal output by two system clock sources, performing a first time-division processing on the first preprocessing signal to obtain a first time-division signal, and performing a second time-division processing on the second preprocessing signal to obtain a second time-division signal according to a preset strategy, performing multiplexing processing on the first time-division signal and the second time-division signal to obtain a PWM output signal, wherein an output frequency of the PWM output signal is a default standard clock frequency. The present invention has the advantages that the cycle length of one cycle of a PWM waveform depends on cycles of two preprocessing waveforms and the number of the cycles, such that the output waveform frequency may be calibrated to a desired frequency.

Abstract: The PWM waveform generation device comprises a time-division multiplexing module, wherein the time-division multiplexing module is configured for receiving a first preprocessing signal and a second preprocessing signal output by two system clock sources, performing a first time-division processing on the first preprocessing signal to obtain a first time-division signal, and performing a second time-division processing on the second preprocessing signal to obtain a second time-division signal according to a preset strategy, performing multiplexing processing on the first time-division signal and the second time-division signal to obtain a PWM output signal, wherein an output frequency of the PWM output signal is a default standard clock frequency. The present invention has the advantages that the cycle length of one cycle of a PWM waveform depends on cycles of two preprocessing waveforms and the number of the cycles, such that the output waveform frequency may be calibrated to a desired frequency.

Abstract: The present invention relates to engineered chimeric guide RNAs and their uses in systems and methods for gene expression perturbation in eukaryotic cells. Furthermore, the invention also relates to methods for generating a modified T cell using the engineered chimeric guide RNAs of the present application, and to the obtained modified T cell. Also included are methods and pharmaceutical compositions comprising the modified T cell for adoptive therapy and treating a condition, such as cancer, infections or autoimmune diseases.

Abstract: Methods, apparatuses, and storage mediums are provided for acquiring a legitimate installation package the field of computer technology. The method includes: acquiring characteristic information of a first installation package; sending the characteristic information to an authentication server; receiving a differential package fed back by the authentication server; and combining the differential package with the first installation package to obtain a legitimate installation package. The present disclosure solves the problem in the prior art that a user can only download a legitimate installation package of an application again to re-install the application when the user finds that malicious codes are implanted in an installed installation package of the application.

Abstract: The disclosure relates to a method and device for playing videos. The method includes playing a first video in a video window; receiving a first instruction to switch windows; transferring the first video from the video window to a floating window in response to receiving the first instruction; receiving a second instruction to play a second video; and closing the floating window in response to receiving the second instruction.

Abstract: Methods, apparatuses, and storage mediums are provided for acquiring a legitimate installation package the field of computer technology. The method includes: acquiring characteristic information of a first installation package; sending the characteristic information to an authentication server; receiving a differential package fed back by the authentication server; and combining the differential package with the first installation package to obtain a legitimate installation package. The present disclosure solves the problem in the prior art that a user can only download a legitimate installation package of an application again to re-install the application when the user finds that malicious codes are implanted in an installed installation package of the application.