Abstract: Provided are a dual-luciferase reporter gene detection system and use thereof. The detection system includes Gaussia luciferase, Pleuromamma xiphias luciferase, and a substrate. The substrate is coelenterazine or a derivative thereof. Compared with other dual-luciferase reporter gene detection systems, the present dual-luciferase reporter gene detection system produces stronger signals and requires simple reaction conditions.

Abstract: Provided are a method for performing position labeling of nucleic acid molecules, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.

Abstract: A method for sequencing polynucleotides by using nanopores. The present invention relates to, in particular, a method for sequencing polynucleotides by means of covalently binding a polynucleotide binding protein and a nanopore.

Abstract: The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same.

Abstract: The present application relates to transcriptome sequencing and biomolecule space information detection. Specifically, the present application relates to a method for positioning and labeling a nucleic acid molecule, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

Abstract: Provided in the present disclosure is a method for sequencing a double-stranded target polynucleotide. The method can keep ATP at a relatively constant concentration during sequencing, so that the sequencing rate can be better kept stable and unchanged. Further provided in the present disclosure is a kit for sequencing a double-stranded target polynucleotide, the kit including a transmembrane pore in the membrane, a helicase, an ATP-generating enzyme and an ATP-generating substrate.

Abstract: A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.

Abstract: The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.

Abstract: A membrane forming device and a membrane forming method are provided. The membrane forming device includes: a base (10); a main body (20) arranged to the base (10) and the main body (20) having a recess; and an opening member (30) arranged inside a recess port on an end of the recess away from the base (10), and a hollow portion (32) of the opening member (30) being configured to form a amphipathic molecular membrane (80), wherein a cross-sectional area of the hollow portion (32) of the opening member (30) is smaller than that of the accommodating chamber (21).

Abstract: Provided are a method for positioning and labeling a nucleic acid molecule, and a method for constructing a nucleic acid molecule library for single-cell transcriptome sequencing, which relate to the technical fields of single-cell transcriptome sequencing and biomolecular space information detection. Further provided are a nucleic acid molecule library constructed by using the method, and a kit for implementing the method.

Abstract: A DNA library sequence for increasing the number of chip probes and an application thereof. The DNA library sequence comprises two or more position information labeling sequences and three or more fixed sequences, wherein the position information labeling sequences and the fixed sequences are arranged at intervals. The DNA library sequence increases the number of probes of a chip used for spatiotemporal omics, and then a capture number of transcript genes is increased. The present invention has a very high application prospect.

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

Abstract: A microfluidic device and a microfluid detection device, comprising a body (100) and a liquid channel (201) provided within the body (100); a fluid input port (103) communicated with the liquid channel (201) is also provided on the body (100), and an arrangement position of the fluid input port (103) does not include a sensing area (301). The microfluidic device and the microfluidic detection device are easy to operate, and can effectively solve the problem of introducing bubbles due to directly opening a liquid injection hole on the sensing area (301).

Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.