Abstract: A miR-520b sequence for inhibiting cancer growth and spread includes miR-520b sequence for inhibiting cancer growth and spread wherein the miR-520b sequence is 5?-AAAGUGCUUCCUUUUAGAGGGtt-3? of SEQ ID NO:2. A medicine for inhibiting cancer growth and spread includes a medicinal preparation including miR-520b substrate and medical agent wherein the miR-520b substrate is miR-520b sequence and has concentration of 50 ?M, and the miR-520b sequence is 5?-AAAGUGCUUCCUUUUAGAGGGtt-3? of SEQ ID NO:2.

Abstract: A method of using sequence of antagomiR for developing medicine for inhibiting oral cancer cells from spreading is provided with the steps of combining a sequence of antagomiR with active ingredient to prepare a medicine which is configured to administer to oral cavity grown with oral cancer cells so that both miR-196a and miR-196b of the sequence of antagomiR are configuring of developing medicine for curing oral cancer cells. The miR-196a has a sequence of 5?-UAGGUAGUUUCAUGUUGUUGGG-3?(SEQ.ID.NO.1). The miR-196b has a sequence of 5?-UAGGUAGUUUCCUGUUGUUGGG-3?(SEQ.ID.NO.2).

Abstract: A method of using miR-196a and miR-196b as biomarkers for oral cancer detection is provided with the steps of analyzing a sample from each of a plurality of human beings in terms of miR-196a and miR-196b wherein the miR-196a has a sequence of SEQ ID NO: 1 and miR-196b has a sequence of SEQ ID NO: 2; and detecting one of the human beings to have oral cancer if intensity of either miR-196a or miR-196b of the sample belonging to the human being is higher than a predetermined value.