Abstract: A plant sterility-related gene is disclosed, in particular to a dominant male sterility-related gene from rice and application of an encoding protein thereof in creating a rice dominant male sterile line. The present invention provides a rice dominant male sterile gene SMS and application thereof. The rice dominant male sterility-related gene SMS may be used for creating a new dominant male sterile line, thereby strengthening use of the advantages of a rice hybrid. The TO generation of transgenic plant line with overexpression of the rice dominant male sterility related gene SMS shows pollen abortion or no pollen, which indicates that the transgenic plant overexpressing this gene can obtain a new sterile material. Creation of the dominant male sterile line can promote wide application of a recurrent selection breeding method, enrich the genetic diversity of a new breeding variety, and solve the problem of a single variety of the rice.

Abstract: The present invention provides a Chlorella variabilis-derived phosphomannose isomerase gene, herein named ChloPMI. The present invention also provides a prokaryotic expression vector comprising ChloPMI, which can be used for identifying mannose metabolic activity of ChloPMI protein. Further, the present invention provides an expression cassette and a plant expression vector comprising ChloPMI, and a use of the expression cassette and the expression vector in genetic transformation of plants. According to the present invention, the transformation of rice cells is successfully achieved with the plant expression vector constructed from the ChloPMI gene using mannose as a selection agent. According to the present invention, a plant-derived phosphomannose isomerase gene is successfully separated and cloned from Chlorella variabilis.