Patents by Inventor Yae Ito

Yae Ito has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for production of recombinant human alpha-galactosidase A from material containing contaminant host cell proteins by chromatography
    Patent number: 10385325
    Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: August 20, 2019
    Assignee: JCR PHARMACEUTICALS CO., LTD.
    Inventors: Tsuyoshi Fukui, Atsuko Kawasaki, Yukichi Hatano, Yae Ito, Kazutoshi Mihara, Atsushi Sugimura
  • ALPHA-GALACTOSIDASE A FROM MATERIAL CONTAINING CONTAMINANT HOST CELL PROTEINS
    Publication number: 20180016564
    Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.
    Type: Application
    Filed: January 21, 2016
    Publication date: January 18, 2018
    Inventors: Tsuyoshi FUKUI, Atsuko KAWASAKI, Yukichi HATANO, Yae ITO, Kazutoshi MIHARA, Atsushi SUGIMURA
  • Method for production of recombinant human FSH
    Patent number: 7939296
    Abstract: Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.
    Type: Grant
    Filed: May 15, 2009
    Date of Patent: May 10, 2011
    Assignee: JCR Pharmaceuticals Co., Ltd.
    Inventors: Atsushi Sugimura, Katsuya Daimon, Kazutoshi Mihara, Yae Ito
  • Method for Production of Recombinant Human FSH
    Publication number: 20090291473
    Abstract: Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.
    Type: Application
    Filed: May 15, 2009
    Publication date: November 26, 2009
    Applicant: JCR PHARMACEUTICALS CO., LTD.
    Inventors: Atsushi Sugimura, Katsuya Daimon, Kazutoshi Mihara, Yae Ito