Abstract: Determining a biological condition in a mammal by using a cartridge, having fluidic open channels, sealable after receiving a fluid specimen, passing any of the specimen through any of the channels, contacting any reagent stored in a chamber with the specimen in a reaction chamber inducing a reaction and forming a reaction product, a mechanical controller including first urging means applying a force externally onto the chamber to release the reagent, second urging means applying a removable force onto the channels thereby inducing fluidic movement in a first direction in the channels and upon removal of the force causing fluidic movement in an opposite direction, alignment means aligning a reading channel on the cartridge for a detection to take place, an optical reader detecting the reaction product in the reading channel, and a processor receiving data from the optical reader and processing the data to determine the biological condition.

Abstract: The present invention provides kits, apparatus and methods for determining a biological condition in a mammalian subject, the method includes incubating a specimen from a patient with at least one composition in a kit for a predetermined period of time to form at least one reaction product, when the subject has said biological condition, and receiving an indication of the at least one reaction product responsive to at least one reporter element in the kit thereby providing the indication of the biological condition in the subject.