Abstract: Water soluble, polyethylenimine polymers, conjugated to one or more active moiety are taught. Also taught are methods of making same, kits comprising same, and use of same in immunoassay systems.

Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.

Abstract: A method is provided for determining active plasminogen activator inhibitor—Type 1 (PAI-1) in a biological fluid, the method comprising the steps of (i) providing a sample of a biological fluid; and (ii) measuring the amount of PAI-1/multimeric vitronectin complex in the sample to determine active PAI-1 in the sample. A kit for carrying out the method is also provided.

Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.

Abstract: Water soluble, polyethylenimine polymers, conjugated to one or more active moiety are taught. Also taught are methods of making same, kits comprising same, and use of same in immunoassay systems.

Abstract: Caged compounds, i.e. synthetic chemical compounds whose chemical or biochemical activity is normally controlled by photolysis to release an active moiety to participate in a chemical or biochemical reaction, are subjected to electrical current instead of light to release the active moiety. The process is especially useful in chemiluminescent biochemical assays, where it is required to measure the light output in order to quantify the analyte under measurement, since avoids confusion between input energy to trigger the reaction, and light output for measurement purposes.

Abstract: There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferase bioluminescent combination, together with an inactive, caged trigger compound such as a cofactor, is subjected to photonic radiation, so as to release the trigger compound in active form, and thereby cause substantially instantaneous reaction of the active trigger compound so released with the luciferase combination, to induce photonic emission which can be detected and measured.