Abstract: Disclosed herein are methods and systems for evaluating and modeling fibrous structures from one or more images. The methods and systems allow for robust, independent, and accurate quantification of fiber orientation in complicated structures, such as the undulating, interweaving, and multidirectional fibers of the human cornea. In addition, the methods and systems can be used to study, repair, and perform quality control on existing biological and industrial structures that include fibers (e.g., carbon nanotubes). Some embodiments can be used to predict the properties (e.g., strength, contrast, and material degradation) of and engineer new biological and industrial structures with fibers (e.g., synthetic corneas).