Abstract: Use of a miR-34b in regulating lipid metabolism of a Shandong Black cattle is provided in the present disclosure, and belongs to the technical field of molecular biological breeding. A miR-34b can be used in breeding of the Shandong Black cattle to screen an individual with a low miR-34b level as a high-quality calf. Experiments have shown that the miR-34b can regulate proliferation and growth of an adipose tissue of the Shandong Black cattle, thereby regulating a birth weight of the calf. The miR-34b can affect proliferation and growth of adipocytes of the Shandong Black cattle, thereby affecting growth of fetal cattle. In breeding, the individual with a low miR-34b level should be selected as a high-quality calf of the Shandong Black cattle. Therefore, a novel direction is provided for breeding a high-quality beef Shandong Black cattle in the present disclosure.

Abstract: A numerical simulation method for characterizing fluid channeling along large-aperture fractures of reservoirs relates to the field of petroleum reservoir development research and computational fluid dynamics. The conventional method can not effectively represent the rapid fluid channeling along large-aperture fractures. Aiming at solving the above technical problem, an advanced method is provided in the present invention. In the invented method, the geometric similarity and hydraulic similarity treatments of large-aperture fractures can be made simultaneously, moreover, the traditional numerical simulation software was improved to be an unstructured grid simulator. Therefore, the method of the present invention can effectively simulate the rapid fluid channeling along large-aperture fractures, consequently it can ensure the reliability of the simulation results and provide reasonable reference for the adjustment and optimization of oil field development plans.

Abstract: A numerical simulation method for characterizing fluid channeling along large-aperture fractures of reservoirs relates to the field of petroleum reservoir development research and computational fluid dynamics. The conventional method can not effectively represent the rapid fluid channeling along large-aperture fractures. Aiming at solving the above technical problem, an advanced method is provided in the present invention. In the invented method, the geometric similarity and hydraulic similarity treatments of large-aperture fractures can be made simultaneously, moreover, the traditional numerical simulation software was improved to be an unstructured grid simulator. Therefore, the method of the present invention can effectively simulate the rapid fluid channeling along large-aperture fractures, consequently it can ensure the reliability of the simulation results and provide reasonable reference for the adjustment and optimization of oil field development plans.