Abstract: This invention relates to the finding that novel splice switching oligonucleotides can correct splicing mutations. Moreover, the invention relates to using the novel splice switching oligonucleotides to correct a 1811+1.6kb G>T (c. 1679+1643G>T). 1811+1.6kb A>G (c. 1679+1634A>G), 1811+1.6kb T>A (c. 1679+1650T>A), or W1282X (c.3846G>A) mutation in a pre-mRNA produced from the human CFTR gene and methods of using the same for treatment of cystic fibrosis (CF) in a subject.

Abstract: This application discloses a device and a method for culturing Geobacter that produces ultra-high conductivity bio-nanowires. The device comprises a tank, at least two liquid color sensors, at least two dissolved oxygen sensors, a liquid level sensor, and a stirring paddle. The top of the tank is provided with an air valve; an upper part of the tank is provided with a feed inlet, a lower part is provided with a culture medium outlet, and the bottom of the tank is provided with a discharge outlet. This application can recycle ferrous citrate, add substrates multiple times, cultivate Geobacter that grow ultra-high conductivity bio-nanowires in batches, and achieve the amplification and cultivation of a large number of Geobacter metallireducens in the same volume.

Abstract: This invention relates to the finding that novel splice switching oligonucleotides can correct splicing mutations. Moreover, the invention relates to using the novel splice switching oligonucleotides to correct the c.1167+1262A?G mutation in a pre-mRNA produced from the human CCDC39 gene and methods of using the same for treatment of primary ciliary dyskinesia (PCD) in a subject.