Abstract: The disclosure discloses a magnetic stimulation method with a controllable induced field direction, and belongs to the technical field of noninvasive neural regulation. The method includes the following steps: S100, determining currents i1j, i2j, i3j, j=1, 2, . . . , n required to respectively generate unit vector electric fields at a target point Pt, when the currents i1j, i2j, i3j, are respectively applied through a coil j of a magnetic stimulation coil group, n?3 and n being an integer; S200, decomposing an electric field E required at the target point Pt to three electric field components E1, E2, E3; S300, calculating currents I1j, I2j, I3j that respectively generate the electric field components E1, E2, E3 at the target point Pt, I1j=E1i1j, I2j=E2i2j, I3j=E3i3j; S400, generating the electric field E at the target point Pt by applying a current Ij=I1j+I2j+I3j=E1i1+E2i2, I3j+E3i3; through the coil j, j=1, 2, . . . , n.

Abstract: Provided are a clearing and expansion method and an imaging method for biological tissue. The clearing and expansion method for biological tissue comprises: (1) degreasing a fixed biological tissue sample; (2) soaking the degreased biological tissue sample in a solution of gel monomer molecules, such that the monomer molecules permeate into the degreased biological tissue sample; (3) inducing a polymerization reaction of the monomer molecules permeating into the biological tissue sample so as to form a polymer gel; and (4) placing the biological tissue sample which has been subjected to a gelation treatment into water for expansion. The clearing and expansion method for biological tissue avoids the sample treatment steps of heating and enzymatic digestion that cause fluorescent quenching and the destruction of biological tissue, and thus has the advantages of high retention of endogenous fluorescent proteins, high mechanical strength of expanded biological tissue, a short sample treatment time, etc.

Abstract: A degreasing composition for clearing biological tissue, a use in the preparation of a degreasing reagent, a degreasing reagent, a clearing kit comprising the degreasing composition or the degreasing reagent, a biological tissue clearing treatment method using the degreasing reagent, and an imaging method for clearing biological tissue comprising the steps of the biological tissue clearing treatment method. The degreasing composition comprises urea, N-butyldiethanolamine and Triton X-100, wherein the mass ratio of urea, N-butyldiethanolamine and Triton X-100 is (0.6-5):(0.3-3):1. The degreasing composition has a fast degreasing speed, good degreasing capabilities for large-volume biological samples, and may retain the structural features of biological tissues well from the cellular level to the subcellular level.

Abstract: A biological tissue sample imaging method, comprising: (1) put a magnetic material plate, a gel precursor solution and a biological tissue sample in an embedding container, and then forming a gel to obtain a gel body, in which the biological tissue sample, and a porous plate having ferromagnetism are embedded; and (2) mount the gel body on the sample holder magnetic base of an imaging microscope, and image.

Abstract: The disclosure discloses a magnetic stimulation method with a controllable induced field direction, and belongs to the technical field of noninvasive neural regulation. The method includes the following steps: S100, calculating currents i1j, i2j, i3j, j=1, 2, . . . n required to be made to generate a unit-direction vector electric field at a target point Pt, the same below; S200, decomposing a vector electric field E required at the target point to three fundamental vector directions to obtain electric field components E1, E2, E3; S300, calculating currents that may generate the electric field components E1, E2, E3 at the target point, I1j=E1i1j, I2j=E2i2j, I3j=E3i3j; S400, superimposing the currents of the three energization modes to obtain a resultant current Ij=I1j+I2j+I3j=E1i1j+E2i2j+E3i3j to be made of each coil of a coil group, that is, generating a required electric field E at the target point for specific directional simulation.