Patents by Inventor Yan-Ping Shih

Yan-Ping Shih has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression system for producing protein having a N-terminal pyroglutamate residue
    Patent number: 9856483
    Abstract: Disclosed herein is an expression system and uses thereof. The expression system comprises two vectors that express two fusion proteins and a glutaminyl cyclase (QC) with E45Q mutation, so as to autonomously produce a target protein having a N-terminal pyroglutamate (pGlu) residue in a host cell. The first fusion protein is composed of a maltose binding protein (MBP) and a tobacco etch virus protease (TEVP); and the second fusion protein includes in sequence, a thioredoxin, a S-tag, a linker having a TEVP recognition site therein, a target protein, and a (His)-6-tag. The second fusion protein is cleaved by the TEVP that is expressed by the first fusion protein, and then catalyzed by QC with E45Q mutation so as to autonomously produce the target protein having a N-terminal pGlu residue in the host cell.
    Type: Grant
    Filed: March 18, 2015
    Date of Patent: January 2, 2018
    Inventors: Andrew H. -J. Wang, Kai-Fa Huang, Yan-Ping Shih
  • EXPRESSION SYSTEM FOR PRODUCING PROTEIN HAVING A N-TERMINAL PYROGLUTAMATE RESIDUE
    Publication number: 20160273015
    Abstract: Disclosed herein is an expression system and uses thereof. The expression system comprises two vectors that express two fusion proteins and a glutaminyl cyclase (QC) with E45Q mutation, so as to autonomously produce a target protein having a N-terminal pyroglutamate (pGlu) residue in a host cell. The first fusion protein is composed of a maltose binding protein (MBP) and a tobacco etch virus protease (TEVP); and the second fusion protein includes in sequence, a thioredoxin, a S-tag, a linker having a TEVP recognition site therein, a target protein, and a (His)-6-tag. The second fusion protein is cleaved by the TEVP that is expressed by the first fusion protein, and then catalyzed by QC with E45Q mutation so as to autonomously produce the target protein having a N-terminal pGlu residue in the host cell.
    Type: Application
    Filed: March 18, 2015
    Publication date: September 22, 2016
    Inventors: Andrew H. -J. Wang, Kai-Fa Huang, Yan-Ping Shih
  • Kit for producing recombinant tag-cleavable fusion proteins via at least two different expression vectors allow protein production in two different species host cells
    Patent number: 9206431
    Abstract: This invention features a kit containing multiple expression vectors for producing tag-cleavable fusion proteins in various expression systems, or for producing fusion proteins in E. coli inclusion bodies.
    Type: Grant
    Filed: September 27, 2007
    Date of Patent: December 8, 2015
    Assignee: Academia Sinica
    Inventors: Po-Huang Liang, Hui-Min Wang, Yan-Ping Shih
  • Protein expression system involving mutated severe respiratory syndrome-associated coronavirus 3C-like protease
    Patent number: 8053222
    Abstract: A mutated severe acute respiratory syndrome-associated coronavirus 3C-like protease and use thereof for cleaving a protein that includes a cleavage site recognizable by the mutated protease to yield a polypeptide fragment of interest.
    Type: Grant
    Filed: February 12, 2009
    Date of Patent: November 8, 2011
    Assignee: Academia Sinica, Taiwan
    Inventors: Po-Huang Liang, Chih-Jung Kuo, Yan-Ping Shih
  • Protein Expression System Involving Mutated Severe Respiratory Syndrome-Associated Coronavirus 3C-Like Protease
    Publication number: 20100203582
    Abstract: A mutated severe acute respiratory syndrome-associated coronavirus 3C-like protease and use thereof for cleaving a protein that includes a cleavage site recognizable by the mutated protease to yield a polypeptide fragment of interest.
    Type: Application
    Filed: February 12, 2009
    Publication date: August 12, 2010
    Applicant: Academia Sinica
    Inventors: Po-Huang Liang, Chih-Jung Kuo, Yan-Ping Shih
  • Producing a Target Protein Using Intramolecular Cleavage by TEV Protease
    Publication number: 20100035300
    Abstract: A cis-TEVP fusion protein including a TEVP protease, a TEVP cleavage site and a target protein provide a platform for expression of the target protein. A trans-TEVP fusion protein including a TEVP cleavage site and a target protein, the amino-terminal portion of the target protein adjacent to the C-terminal portion of the TEVP cleavage site, the amino acidic residue in position P2 of the TEVP cleavage site being a Valine also produces the target protein by the same process. A cis-TEVP fusion protein system comprising the first fusion protein and a suitable host cell; a trans-TEVP fusion protein system comprising the second fusion protein and a suitable host cell; associated methods to produce target proteins, and kits of parts are also disclosed herein.
    Type: Application
    Filed: February 27, 2006
    Publication date: February 11, 2010
    Inventors: Andrew H.-J. Wang, Ting-Fang Wang, Yan-Ping Shih, Hui-Chung Wu, Su-Ming Hu
  • EXPRESSION VECTORS FOR PRODUCING TAG-CLEAVABLE FUSION PROTEINS IN MULTIPLE EXPRESSION SYSTEMS
    Publication number: 20090087895
    Abstract: This invention features a kit containing multiple expression vectors for producing tag-cleavable fusion proteins in various expression systems, or for producing fusion proteins in E. coli inclusion bodies.
    Type: Application
    Filed: September 27, 2007
    Publication date: April 2, 2009
    Applicant: Academia Sinica
    Inventors: Po-Huang Liang, Hui-Min Wang, Yan-Ping Shih