Abstract: The invention relates to cell biology and regenerative medicine, specifically to a culture method and application for porcine limbal epithelial stem cells. The method involves pretreatment of cells, coating of culture dishes, centrifugation, culturing, passaging, and identification by immunofluorescence staining. The cultured cells are applicable in repairing corneal diseases, including limbal stem cell deficiency. During repair, the cells can be combined with biological materials to form a stem cell engineering carrier for implantation at the injury site. The separation and culture method is simple, yields a large number of cells, and is suitable for use with porcine eyeballs. Limbal epithelial stem cells cultured in vitro form distinct cell clusters, referred to as stem cell islands. The obtained cells exhibit phenotypes including BMI1+, ?Np63?+, CK14+, and ABCG2+.

Abstract: A gap inspection system is configured to inspect global gap data between mask and substrate, comprising: support frame; mask frame comprising autofocus module for real-time monitoring gap data between mask and substrate; wafer stage module comprising wafer chuck for adjusting position and orientation of substrate; front-side surface topography inspection module, mounted on upper substrate of support frame for inspecting the substrate, to acquire primary surface topography parameters; folding surface topography inspection module, switchable between inspection position and retract position for inspecting mask, to acquire secondary surface topography parameters; and control system, configured to acquire position parameters of mask and substrate and calibration parameters of front-side surface topography inspection module and folding surface topography inspection module, and configured to calculate global gap data between mask and substrate in conjunction with position parameters, calibration parameters, primary

Abstract: Alignment method of mask and substrate includes: summing grayscale values in mark image row by row or column by column respectively, to obtain first and second projection summation curves of rows and columns; calculating first mean value and standard deviation of center coordinates of mask mark, and second one of substrate mark based on above projection summation curves; extracting image contours of mark image, and adding horizontal or vertical processing window to image contours; extracting first contour grayscale curve of each row in horizontal processing window or second one of each column in vertical processing window; calculating third mean value and standard deviation of center coordinates of mask mark, and fourth one of substrate mark according to first or second contour grayscale curve; performing statistical fusion on four mean values and four standard deviations to obtain alignment deviation; and aligning mask and substrate according to alignment deviation.

Abstract: The invention relates to bioinformatics technology and specifically to an analysis method of a spatial transcriptome-based 4D-printed stem cell scaffold for enhancing diabetic skin injury healing. Spatial transcriptome sequencing is performed on tissues from both the PBS group and the combined treatment group. Cell clustering and annotation identify main cell types, followed by visualization of their spatial distributions in both groups. The proportions of various cell types are statistically quantified and plotted. Cell subsets exhibiting significant differential gene expression are enriched and verified. Using a 4D-printed stem cell scaffold combined with a piRNA inhibitor and stem cell therapy, the method reveals spatial distribution and dynamic cellular changes during wound healing. It identifies precise cellular locations and gene expression patterns within tissues, providing critical insights into the biological processes by which biomaterials promote diabetic injury repair.

Abstract: The present invention relates to cell biology and regenerative medicine, specifically to a method for isolating and culturing porcine endometrial mesenchymal stem cells (PEMSCs) and their applications. PEMSCs are sourced from uterine tissue of pigs, providing a streamlined process yielding a substantial quantity of cells. Cultured PEMSCs exhibit CD34?, CD11b?, CD44+, and CD105+ phenotypic markers and can differentiate into osteocytes, adipocytes, and chondrocytes in vitro. The method employs type II collagenase to degrade extracellular matrix collagen and DNase I to break down DNA, reducing cell aggregation. This combination ensures efficient tissue digestion and high-quality single-cell suspensions. Applications include tissue repair for intrauterine adhesions, skin injuries, and vascular damage. The repair involves combining PEMSCs with biomaterials and implanting them into lesion sites.

Abstract: The present invention belongs to the field of biomedical technology, and specifically relates to a new application for piR-hsa-164586 and MYH9, based on the interaction between piR-hsa-164586 and MYH9: piR-hsa-164586 regulates the expression of MYH9 and promotes the metastasis of NSCLC, piR-hsa-164586 and MYH9 can be used as a therapeutic target for NSCLC, and have become a potential therapeutic drug, wherein the MYH9 is obtained by RNA pulldown assay and verified by protein mass spectrometry, molecular docking and RIP assays, the piR-hsa-164586 can positively regulate the expression of MYH9, and verified by performed qRT-PCR, western blotting and tissue immunofluorescence staining assays on the piR-hsa-164586 NC group, piR-hsa-164586 KD group, and piR-hsa-164586 OE group. The interaction between piR-hsa-164586 and MYH9 can up-regulate the expression of MYH9 in NSCLC cell lines, the verification experiment process was confirmed by Transwell experiment after knockdown of piR-hsa-164586 and MYH9 respectively.

Abstract: An illumination compensation method, comprising: acquiring working gaps between an imaging film stack and a mask in an exposure imaging system; calculating imaging light field intensities of a photoresist layer in the imaging film stack corresponding to the different working gaps, and, constructing a functional dependence of imaged pattern critical dimension in the photoresist layer of the imaging film stack on the working gap based on said imaging light field intensity critical dimension; calculating compensation parameters required for illumination light according to the said functional dependence of imaged pattern critical dimension on working gap; and, performing illumination compensation on the exposure imaging system according to the compensation parameters.

Abstract: A method for modifying a polymer membrane is disclosed and includes: pre-wetting a polymer membrane using a crosslinking agent solution, where the crosslinking agent solution includes a first crosslinking agent and a second crosslinking agent; irradiating the pre-wetted polymer membrane to initiate a crosslinking reaction; and rinsing and drying the polymer membrane after the crosslinking reaction to obtain a modified polymer membrane. The method for modifying the polymer membrane uses two cross-linking agents to modify the polymer membrane, thereby forming a 3D network on the surface and within the bulk of the polymer membrane to obtain a modified polymer membrane. The modified polymer membrane has low protein adsorption, caustic stability, autoclave sterilization stability, and gamma sterilization stability, while retaining the overall mechanical properties to meet the pleatability requirement for filter manufacturing.

Abstract: A reversable attachment system includes an adhesion layer, an inter-substrate bond structure, a mating layer and an extension actuator. The adhesion layer is configured to attach to a first substrate. The inter-substrate bond structure is coupled to the adhesion layer. The mating layer is configured to attach to a second substrate. The extension actuator is configured to attach to the second substrate and expand in response to an absorption of a gas. The inter-substrate bond structure is configured to form an initial thermocompression bond with the mating layer in response to an applied pressure and an applied heat. The expansion of the extension actuator in response to absorbing the gas detaches the inter-substrate bond structure from the mating layer.

Abstract: Provided herein are compositions and methods using a therapeutic agent targeting mTORCI and a therapeutic agent targeting MDK for treating a Tuberous Sclerosis Complex (TSC)-associated disease, e.g., Angiomyolipoma (AML) and lymphangioleiomyomatosis (LAM), or for treating sporadic LAM/AML. Also provided are methods of identifying subjects for treatment, e.g., with checkpoint inhibitors.

Abstract: An illumination compensation method, comprising: acquiring working gaps between an imaging film stack and a mask in an exposure imaging system; calculating imaging light field intensities of a photoresist layer in the imaging film stack corresponding to the different working gaps, and, constructing a functional dependence of imaged pattern critical dimension in the photoresist layer of the imaging film stack on the working gap based on said imaging light field intensity critical dimension; calculating compensation parameters required for illumination light according to the said functional dependence of imaged pattern critical dimension on working gap; and, performing illumination compensation on the exposure imaging system according to the compensation parameters.

Abstract: Disclosed are compositions including a pharmaceutical agent assembled with a lipid composition, wherein the lipid composition comprises a lipidoid having structural Formula (I): or a pharmaceutically acceptable salt thereof, wherein Ra, Rb1, Rb2, Rb3, Rb4, n1 and n2 are as described herein.

Abstract: A transferring robot is disclosed in the disclosure. The transferring robot includes a movable chassis configured to move along a path among adjacent warehouse storage containers; a temporary storage shelf provided on the movable chassis, the temporary storage shelf being configured to store a target case; a fetching assembly provided on the movable chassis, the fetching assembly being configured to extend or retract horizontally relative to the movable chassis, so as to realize fetching and placing of the target case between the storage containers and the temporary storage shelf, where a direction along which the fetching assembly extends or retracts horizontally is perpendicular to a moving direction. A warehousing and logistics system includes the foregoing transferring robot. The disclosure further discloses a warehousing system and an item transferring method.

Abstract: A transferring robot is disclosed in the disclosure. The transferring robot includes a movable chassis configured to move along a path among adjacent warehouse storage containers; a temporary storage shelf provided on the movable chassis, the temporary storage shelf being configured to store a target case; a fetching assembly provided on the movable chassis, the fetching assembly being configured to extend or retract horizontally relative to the movable chassis, so as to realize fetching and placing of the target case between the storage containers and the temporary storage shelf, where a direction along which the fetching assembly extends or retracts horizontally is perpendicular to a moving direction. A warehousing and logistics system includes the foregoing transferring robot. The disclosure further discloses a warehousing system and an item transferring method.

Abstract: A method for determining an IP address of a 5G user terminal is disclosed. A SMF determines a target DNN matching the SMF and a type of a terminal address pool for the target DNN. For dynamic configuration, the SMF configures a first UPF and a second UPF in a load balancing mode, and the first UPF or the second UPF selects an IP address from respective IP address pools as a dynamic IP address of the user terminal accessing the target DNN. For fixed acquisition, the SMF configures the first UPF and the second UPF in an active-standby mode, and the first UPF or the second UPF as an active apparatus determines a fixed IP address of the user terminal accessing the target DNN from the respective IP address pool.

Abstract: A reversable attachment system includes an adhesion layer, an inter-substrate bond structure, a mating layer and an extension actuator. The adhesion layer is configured to attach to a first substrate. The inter-substrate bond structure is coupled to the adhesion layer. The mating layer is configured to attach to a second substrate. The extension actuator is configured to attach to the second substrate and expand in response to an absorption of a gas. The inter-substrate bond structure is configured to form an initial thermocompression bond with the mating layer in response to an applied pressure and an applied heat. The expansion of the extension actuator in response to absorbing the gas detaches the inter-substrate bond structure from the mating layer.

Abstract: The present disclosure provides a spiral blade, a grinding component and a food processor. The spiral blade includes: a spiral blade body, and the spiral blade body includes a feeding area used for feeding, a grinding area connected with the feeding area and a discharging area connected with the grinding area; and a plurality of spiral ribs are arranged on the grinding area, and a cleaning structure is arranged on the discharging area. The spiral blade is applied to the food processor and is only used for grinding food rather than extruding juice and discharging residues, therefore its structure is relatively simple, meanwhile, as a cleaning structure is arranged on the discharging area, the cleaning structure can be used for pushing the crushed food to a predetermined area.

Abstract: The present disclosure provides a vacuum pumping device and a food processor. The vacuum pumping device includes an upper cover; a lower cover configured to cooperated with the upper cover to form a receiving cavity and including a connecting part at a lower portion of the lower cover, a wall of the receiving cavity defining an evacuation hole and an exhaust hole; a mounting support located in the receiving cavity and connected to the upper cover and/or the lower cover; a vacuum pump connected to the mounting support, and including an air inlet communicating with the evacuation hole and an air outlet communicating with the exhaust hole; a control circuit board connected to the mounting support and electrically connected to the vacuum pump; and a power supply device located in the receiving cavity and electrically connected to the control circuit board.

Abstract: Embodiments of the present invention relate to the field of communications technologies, and provide a message processing method and apparatus, to resolve a prior-art problem that intelligence of a notification message processing method is relatively low. In the embodiments of the present invention, an importance level of each application is determined based on a historical use feature of each application, and then a notification manner of a notification message of each application is determined based on the importance level of each application, so that when a notification message is received, the notification message is displayed in the notification manner corresponding to the application to which the notification message belongs. Solutions provided in the embodiments of the present invention are suitable for use during notification message processing.

Abstract: Embodiments provide a fault detection method and device. The method includes obtaining first detection logic, where the first detection logic includes a plurality of detection events and indicates a logical relationship between the plurality of detection events, a single detection event includes identification information of a to-be-detected device, a detection instruction, and identification information of a fault event, and identification information of to-be-detected devices that is in the plurality of detection events is partially the same; in response to the obtained first detection logic, successively executing corresponding detection instructions on to-be-detected devices in single detection events based on the logical relationship between the plurality of detection events, and receiving an execution result returned by the to-be-detected device; and if it is detected that the execution result includes the identification information of the fault event, determining that the fault event exists.