Abstract: The invention relates to methods of using full length amino acid sequence and cDNA sequence of protein human JNK interacting protein (hJ1P1) islet brain 1 (IB1) to treat neurological disorders in human subjects. In an in vitro model of the apoptosis pathway, HJIP-1/IB1 inhibits phosphorylation of the transcription factor c-Jun in a dose-dependent manner. The invention includes antibodies that specifically bind to hJIP-1/IB1.

Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.