Abstract: The invention provides an Escherichia coli for synthesizing L-valine, a construction method and use thereof. The Escherichia coli of the invention is designated as Escherichia coli W3110 and was deposited in China Center for Type Culture Collection (Address: Bayi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022. The recombinant Escherichia coli takes Escherichia coli as a starting strain, and a transcription regulation factor is overexpressed to obtain a recombinant Escherichia coli. The recombinant Escherichia coli for synthesizing L-valine of the invention is fermented in a 5 L fermentor with trace dissolved oxygen to test strains, the yield of L-valine reaches 112 g/L, and the OD of the bacterium is 104.

Abstract: A method for constructing a multistage network includes: deploying a plurality of nodes; constructing the upper network with at least one node of the plurality of nodes; and in response to determining there is an isolated node, determining an intermediate node for the isolated node and adding the isolated node into the upper network or one of the at least one sub-network according to the intermediate node.

Abstract: A method for constructing a large-span station by two-wing open type semi-covered excavation and semi-reverse construction, is divided into three stages of excavation. First, excavate a first-stage inner small foundation pit, then excavate a second-stage annular foundation pit within the first-stage retaining piles and outside the range of the first-stage inner small foundation pit, and finally excavate a third-stage semi-covered excavation foundation pit below the first-stage inner small foundation pit and the second-stage annular foundation pit. By setting graded retaining piles, middle upright post piles, middle top plates, local waist beams and local concrete supports in the soil-rock combination strata, so that the force transfer between the foundation pit enclosure and the station main body structures and the underlying rock layer is clear and reliable, and a stable frame structure is achieved.

Abstract: This disclosure relates to anti-TNFRSF9 (tumor necrosis factor receptor superfamily member 9) antibodies, antigen-binding fragments, and the uses thereof.

Abstract: This application provides a task migration system and method. The system includes a first terminal and a second terminal. The second terminal runs a first application. The first terminal opens a recent task interface after receiving a user operation, where the recent task interface includes an identifier of the second terminal; after receiving a user operation performed on the identifier of the second terminal, displays, in the recent task interface, at least one task card corresponding to an application run by the second terminal in the background; and after receiving a user operation performed on a first task card corresponding to the first application, runs the first application, and displays a first user interface of the first application.

Abstract: A method for constructing a large-span station by two-wing open type semi-covered excavation and semi-reverse construction, is divided into three stages of excavation. First, excavate a first-stage inner small foundation pit, then excavate a second-stage annular foundation pit within the first-stage retaining piles and outside the range of the first-stage inner small foundation pit, and finally excavate a third-stage semi-covered excavation foundation pit below the first-stage inner small foundation pit and the second-stage annular foundation pit. By setting graded retaining piles, middle upright post piles, middle top plates, local waist beams and local concrete supports in the soil-rock combination strata, so that the force transfer between the foundation pit enclosure and the station main body structures and the underlying rock layer is clear and reliable, and a stable frame structure is achieved.

Abstract: Disclosed are a method and a system for forecasting an agricultural product price based on signal decomposition and deep learning. The method includes: S1, obtaining price subsequences by performing a complementary ensemble empirical mode decomposition (CEEMD) on an original price sequence of agricultural products; S2, obtaining a reconstructed sequence based on the price subsequences; S3, obtaining data features of the reconstructed sequence based on the reconstructed sequence; and S4, constructing a Bi-directional Sequence to Sequence (BiSeq2seq) model, and inputting the data features of the reconstructed sequence into a CCS-Bi-directional Sequence to Sequence (CCS-BiSeq2seq) model to obtain a forecasting result.

Abstract: Disclosed is a sex identification specific DNA sequence and a sex identification method of Chinese mitten crab Eriocheir sinensis. The sex identification specific DNA sequence is showed as SEQ ID NO:1. The sex identification method includes following steps:S1, extracting genomic DNA of Chinese mitten crab Eriocheir sinensis; S2, PCR amplification using the genomic DNA as template; an upstream primer for PCR amplification is as shown in SEQ ID No: 2 and a downstream primer is as shown in SEQ ID No: 3; S3, examining amplified products by agarose electrophoresis, and those with specific amplified bands are females, otherwise they are males. The method has high identification accuracy, is simple and easy to operate, and is suitable for sex identification of Chinese mitten crab Eriocheir sinensis at embryonic and larval stages.

Abstract: A blowing-suction machine includes a casing, a suction duct arranged at the front portion of the casing. It further includes a motor and a shredder mechanism that are arranged in the casing in order from rear to front. The front end of the shredder mechanism is connected to the rear end of the suction duct. The shredder mechanism includes blades and a cutting assembly that are arranged in order from rear to front. The blades are arranged on an output shaft of the motor. The cutting assembly is arranged at a rear end of the suction duct for shredding fallen leaves. The cutter cooperates with the secondary cutter such that fallen leaves and rigid trashes can be effectively shredded by the blowing-suction machine. Fallen leaves can be effectively separated from air via the collecting cartridge and the filtering unit, such that the amount of the collected leaves is effectively increased.

Abstract: The present application relates to a method, a system, and intelligent terminal for controlling the operating range of a walking device. The present application resolves the time-consuming and laborious problem caused by installing an electronic fence or performing an artificial wiring before using a mower. In the present application, an electronic fence is not needed, the time and cost of manual installation is reduced and the use efficiency is improved.

Abstract: A blowing-suction machine includes a casing, a suction duct arranged at the front portion of the casing. It further includes a motor and a shredder mechanism that are arranged in the casing in order from rear to front. The front end of the shredder mechanism is connected to the rear end of the suction duct. The shredder mechanism includes blades and a cutting assembly that are arranged in order from rear to front. The blades are arranged on an output shaft of the motor. The cutting assembly is arranged at a rear end of the suction duct for shredding fallen leaves. The cutter cooperates with the secondary cutter such that fallen leaves and rigid trashes can be effectively shredded by the blowing-suction machine. Fallen leaves can be effectively separated from air via the collecting cartridge and the filtering unit, such that the amount of the collected leaves is effectively increased.

Abstract: A stepless speed regulating control mechanism for a mower includes a push handle, a self-propelled lever, a speed regulating cable and a regulating mechanism; the mower has a base and a self-propelled device; the regulating mechanism includes a regulating sleeve and a speed regulating sleeve; the self-propelled lever pulls the regulating sleeve and causes the speed regulating sleeve to move simultaneously with the regulating sleeve, so as to pull the speed regulating cable, and the speed regulating sleeve can move relative to the regulating sleeve, so as to pull the speed regulating cable.

Abstract: A stepless speed regulating control mechanism for a mower, which comprises a push handle, a self-propelled lever, a speed regulating cable and a regulating mechanism; the mower has a base and a self-propelled device; the regulating mechanism comprises a regulating sleeve and a speed regulating sleeve; the self-propelled lever pulls the regulating sleeve and causes the speed regulating sleeve to move simultaneously with the regulating sleeve, so as to pull the speed regulating cable, and the speed regulating sleeve can move relative to the regulating sleeve, so as to pull the speed regulating cable. The stepless speed regulating control mechanism of a mower is not only convenient for speed regulation and for stepless speed regulation, but also reasonable in design and convenient for speed regulation operation without causing confusion and misoperation. Besides, the stepless speed regulating control mechanism is low in cost, and easy to manufacture and operate.