Abstract: A method, system and device for programmed cell freezing is disclosed. The method includes: S1, acquiring a cooling rate k and a temperature distribution function T=a×h2+b×h+c of a heat preservation chamber containing liquid nitrogen; S2, controlling a cell preservation tube to lower in the chamber at a speed of v=(k-b)/2ah, and acquiring temperatures T1 and T2 inside and outside the tube at a same time and at a same height in real time during the lowering of the tube; S3, determining whether a difference ?t between T1 and T2 exceeds a preset temperature difference threshold, if yes, proceeding to S4, and if no, proceeding to S2 until the tube reaches a position corresponding to a preset temperature T0; and S4, controlling the cell preservation tube to stop until the difference ?t between T1 and T2 is less than or equal to the temperature difference threshold, and continuing S2.