Abstract: Provided are an experimental apparatus and method for regulating and controlling the Zeeman transition of magnetic resonance. The method includes: generating a constant background magnetic field by using a magnetic shielding cylinder, a background magnetic field coil, and a constant current source, and controlling a Larmor precession frequency corresponding to the background magnetic field to be equal to a frequency of a sinusoidal signal output by a radio frequency signal source; adjusting the amplitude of a signal output by a radio frequency signal source of a pump-probe atomic magnetometer to set a radio frequency signal to be a ?/2 pulse or a ? pulse; and then controlling the radio frequency signal source to output a series of sinusoidal radio frequency pulse strings having the same initial phase. The Zeeman transition of magnetic resonance is regulated and controlled by adjusting the time interval between the radio frequency pulse signals.