Abstract: A flow-through microfluidic apparatus for cell treatment is provided, including a flow chamber and a three-dimensional (3D) microelectrode array disposed in the flow chamber to produce electrical fields for cell railing, electroporation and/or sorting. The flow chamber includes a sample flow region, a first sheath flow region and a second sheath flow region. The sample flow region has an input allowing a sample flow of cells and exogenous agents to enter the sample flow region, a first output allowing damaged cells to exit from the flow chamber. Viable target cells are dielectrophoretic railed along the plurality of 3D microelectrode units; railing cells are electroporated through electrical treatment to intake/uptake of an exogenous agent; and viable treated cells loaded with exogenous agent are dielectrophoretically sorted from cells damaged during the cell treatment.

Abstract: The present disclosure relates to systems and methods for image storage. The methods may include obtaining a first image of a subject. The methods may further include obtaining a second image of the subject. The second image may include scan status information of the subject. The scan status information may be associated with a status of the subject when the first image is acquired. And The methods may also include storing the second image correspondingly with the first image.

Abstract: A synchronous coefficient of drag alteration method and system for concentrating and purifying nucleic acids from a sample containing unwanted molecules and contaminants are provided. The method includes providing a layer of artificial gel or sieve filled with liquid and into which molecules including nucleic acids are introduced from a sample liquid by applying a steady electric field across a boundary between the artificial gel layer and the sample liquid containing the molecules including nucleic acids; providing a plurality of electrodes spaced apart around circumference of the artificial gel layer and for delivering a pre-programmed sequence of voltage waveforms to establish a primary electric field driving molecules within the artificial gel layer and a secondary electric field altering electrophoretic mobility of molecules within the artificial gel layer. The nucleic acids alone are spatially separated from the unwanted molecules and contaminants to be relatively purified and enriched.

Abstract: The present disclosure is related to systems and methods for subject positioning. The method includes generating a reference subject model of a subject to be scanned by a medical device based on feature information of the subject and a scan protocol of the subject. The method includes generating a target subject model by performing one or more rendering operations on the reference subject model based on at least one rendering parameter associated with an image capturing device. The method includes generating a positioning result by integrating the target subject model with image data related to a scan scene of the subject captured by the image capturing device. The method includes positioning the subject based on the positioning result.

Abstract: In some embodiments, provided is microfluidic devices and methods for sorting particles in a sample. In some embodiments, the microfluidic device comprises a sample inlet for sample loading; at least one reservoir; a sieving array; and at least one outlet for collecting any sorted particles. Other example embodiments are described herein. In certain embodiments, the microfluidic devices and methods provide simple, rapid, efficient and versatile solutions for sorting particles such as extracellular vesicles.

Abstract: A simultaneous phase-shift point diffraction interferometer and method for detecting wave aberration. The interferometer comprises an ideal spherical wave generation module, an optical system to be measured, an image plane mask, a polarization phase shift module, a two-dimensional polarization imaging photodetector and a data processing unit. Single photodetector is adopted to realize simultaneous detection of more than three phase shift interference patterns, and has the advantages that environmental interference suppression, a flexible optical path, high measurement accuracy, and calibration of system errors of the interferometer may be realized.

Abstract: The present disclosure is related to systems and methods for subject positioning. The method includes generating a reference subject model of a subject to be scanned by a medical device based on feature information of the subject and a scan protocol of the subject. The method includes generating a target subject model by performing one or more rendering operations on the reference subject model based on at least one rendering parameter associated with an image capturing device. The method includes generating a positioning result by integrating the target subject model with image data related to a scan scene of the subject captured by the image capturing device. The method includes positioning the subject based on the positioning result.

Abstract: A flow-through microfluidic apparatus for cell treatment is provided, including a flow chamber and a three-dimensional (3D) microelectrode array disposed in the flow chamber to produce electrical fields for cell railing, electroporation and/or sorting. The flow chamber includes a sample flow region, a first sheath flow region and a second sheath flow region. The sample flow region has an input allowing a sample flow of cells and exogenous agents to enter the sample flow region, a first output allowing damaged cells to exit from the flow chamber. Viable target cells are dielectrophoretic railed along the plurality of 3D microelectrode units; railing cells are electroporated through electrical treatment to intake/uptake of an exogenous agent; and viable treated cells loaded with exogenous agent are dielectrophoretically sorted from cells damaged during the cell treatment.

Abstract: The present disclosure relates to systems and methods for image storage. The methods may include obtaining a first image of a subject. The methods may further include obtaining a second image of the subject. The second image may include scan status information of the subject. The scan status information may be associated with a status of the subject when the first image is acquired. And The methods may also include storing the second image correspondingly with the first image.

Abstract: A simultaneous phase-shift point diffraction interferometer and method for detecting wave aberration. The interferometer comprises an ideal spherical wave generation module, an optical system to be measured, an image plane mask, a polarization phase shift module, a two-dimensional polarization imaging photodetector and a data processing unit. Single photodetector is adopted to realize simultaneous detection of more than three phase shift interference patterns, and has the advantages that environmental interference suppression, a flexible optical path, high measurement accuracy, and calibration of system errors of the interferometer may be realized.

Abstract: The present disclosure relates to systems and methods for image storage. The methods may include obtaining a first image of a subject. The methods may further include obtaining a second image of the subject. The second image may include scan status information of the subject. The scan status information may be associated with a status of the subject when the first image is acquired. And The methods may also include storing the second image correspondingly with the first image.

Abstract: Certain embodiments of the invention provide TiO2 nanoshell particles, methods of fabricating TiO2 nanoshell particles, and methods of enriching peptides in a sample using TiO2 nanoshell particles.

Abstract: The present disclosure relates to systems and methods for image storage. The methods may include obtaining a first image of a subject. The methods may further include obtaining a second image of the subject. The second image may include scan status information of the subject. The scan status information may be associated with a status of the subject when the first image is acquired. And The methods may also include storing the second image correspondingly with the first image.

Abstract: Certain embodiments of the invention provide TiO2 nanoshell particles, methods of fabricating TiO2 nanoshell particles, and methods of enriching peptides in a sample using TiO2 nanoshell particles.