Abstract: A recombinant genetic construct is provided that includes at least two expression cassettes. Each cassette encodes for a chain or subunit of a target protein. The genetic construct preferably targets any expressed protein to the secretory pathway of the host cell. An application of present invention is found in expressing the two chains of human insulin through two separate expression cassettes on the same methylotrophic yeast expression vector. Mature, bioactive human insulin molecules are secreted through this method without resorting to any post-translational cleavage process.