Abstract: Disclosed are a pixel layout structure, a display panel, a mask plate assembly. The pixel layout structure includes multiple repeating units arranged in an array. An outer contour of each repeating unit is a rectangle; each repeating unit includes four pixel units, and each pixel unit comprises a first sub-pixel, a second sub-pixel, and a third sub-pixel; the four first sub-pixels are arranged around a center of the repeating unit; an outer contour of the four first sub-pixels includes a first edge, a second edge, a third edge, and a fourth edge; each of the first edge and the third edge is arranged with two second sub-pixels, and each of the second edge and the fourth edge is arranged with two third sub-pixels; or each of the first edge, the second edge, the third edge, and the fourth edge is arranged with a second sub-pixel and a third sub-pixel.