Abstract: In the field of biomedical technology, a PIWI-interacting RNA piR-hsa-211106 is used to prepare a targeted therapeutic drug for inhibiting proliferation of lung adenocarcinoma cells. A mechanism is as follows: after the PIWI-interacting RNA piR-hsa-211106 is constructed into an agonist or a transformant, the agonist or the transformant inhibits a tricarboxylic acid cycle process by down-regulating an expression of a pyruvate carboxylase, inhibits an energy metabolism, promotes apoptosis of the lung adenocarcinoma cells, thus inhibits growth of the lung adenocarcinoma. The PIWI-interacting RNA piR-hsa-211106 directly acts on a target site and does not produce toxic and side effects and an off-target phenomenon. Large amounts of analysis and in-vivo and in-vitro experiments show that the PIWI-interacting RNA piR-hsa-211106 has high credibility and a remarkable treatment effect, and provides a new research direction for anti-tumor therapy of lung adenocarcinoma.

Abstract: Medical material production and preparation, and a preparation method of a 4D chitosan-based thermosensitive hydrogel. First, chitosan is dissolved in acetic acid solution; a chitosan-based thermosensitive hydrogel is printed by a 4D bioprinter and lyophilized after solvent extraction, to obtain lyophilized chitosan; subsequently, aqueous ?-sodium glycerophosphate solution is prepared with ultrapure water and ?-sodium glycerophosphate, and then aqueous carboxymethyl chitosan solution is prepared with ultrapure water and aqueous ?-sodium glycerophosphate solution are charged into and mixed well with aqueous carboxymethyl chitosan solution to prepare a mixture; finally, the lyophilized chitosan is crosslinked with the mixture to obtain the 4D chitosan-based thermosensitive hydrogel. With scientific and reliable principles thereof, the present invention solves a problem that conventional thermosensitive hydrogels have uneven pore sizes, and improves the entrapment efficiency and ability of limbal stem cells.