Abstract: Provided herein are quantitative assays to evaluate duplex unwinding efficiency or strand exchange efficiency during isothermal amplification.

Abstract: Provided herein is the conjugation of an amanita toxin compound to a cell-binding molecule with branched linkers for having better targeted treatment of abnormal cells. It also relates to a branched-linkage method of conjugation of an amanita molecule to a cell-binding ligand, as well as methods of using the conjugate in targets treatment of cancer, infection and autoimmune disease.

Abstract: The present invention relates to a conjugate of a cytotoxic drug/molecule to a cell-binding molecule with a bis-linker (a dual-linker) containing a 2,3-diaminosuccinyl group. It also relates to preparation of the conjugate of a cytotoxic drug/molecule to a cell-binding molecule with the bis-linker, particularly when the drug having functional groups of amino, hydroxyl, diamino, amino-hydroxyl, dihydroxyl, carboxyl, hydrazine, aldehyde and thiol for conjugation with the bis-linker in a specific manner, as well as the therapeutic use of the conjugates.

Abstract: Provided herein are methods, compositions, and kits for detecting a target nucleic acid, such as from a virus, in a biological sample. More specifically, the methods, compositions, and kits described herein describe detection of target nucleic acid from a coronavirus, such as SARS-CoV-2 coronavirus, with non-ionic detergents and isothermal amplification.

Abstract: This invention relates in general to methods of sequencing multiple distinct and separate polynucleotide fragments and regions in a sequential order, such as on a flow cell surface. The invention provides methods that solve prior art problems with regard to sequential sequencing, and that provide advantages including low cost, shorter turn-around-time, high efficiency, and easy implementation.

Abstract: A flexible planar embedded laminated electrode can be manufactured by a method in which an octadecyl trichloro silane connected on the surface of a substrate; a source electrode, a drain electrode and a gate electrode are manufactured using photoetching; mercaptopropyl trimethoxysilane is connected at surfaces of metal electrodes of the source electrode, the drain electrode and the gate electrode; a polydimethylsiloxane is spin-coated on the respective surfaces for metal electrodes of the above electrodes; the gate electrode spin-coated with polydimethylsiloxane is removed from the substrate; oxygen plasma treatments are performed, so as to form hydroxy on the surfaces; the source electrode and the drain electrode are cut, and the gate electrode, the source electrode and the drain electrode are connected to form an integral to obtain the flexible planar embedded laminated electrode.

Abstract: The present invention provides an organic single crystal field effect circuit and method for preparing the same.

Abstract: The present invention discloses a flexible planar embedded laminated electrode and manufacturing method and application thereof.

Abstract: The present invention provides an organic single crystal field effect circuit and method for preparing the same.

Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

Abstract: Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of test and control nucleic acids. Test nucleic acids can be immobilized at multiple locations, such that amplification of either a test nucleic acid or a control nucleic acid provides a captured nucleic acid in a control capture zone.

Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.

Abstract: Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of test and control nucleic acids. Test nucleic acids can be immobilized at multiple locations, such that amplification of either a test nucleic acid or a control nucleic acid provides a captured nucleic acid in a control capture zone.