Patents by Inventor Yanjie Bao
Yanjie Bao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240115691Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: ApplicationFiled: June 30, 2023Publication date: April 11, 2024Inventors: Sean Michael SULLIVAN, Daiki MATSUDA, Kiyoshi TACHIKAWA, Padmanabh CHIVUKULA, Priya Prakash KARMALI, Jared Henry DAVIS, Yanjie BAO, Amit SAGI
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Publication number: 20240115692Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: ApplicationFiled: July 12, 2023Publication date: April 11, 2024Inventors: Sean Michael SULLIVAN, Daiki MATSUDA, Kiyoshi TACHIKAWA, Padmanabh CHIVUKULA, Priya Prakash KARMALI, Jared Henry DAVIS, Yanjie BAO
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Patent number: 11938227Abstract: A method of producing lipid-encapsulated RNA nanoparticles includes flowing an aqueous solution comprising an RNA through a 1st tube having a first inner diameter (ID); the RNA comprises from about 6,000 to about 13,000 nucleotides; flowing an ethanol solution comprising lipids through a 2nd tube having a second inner diameter (ID), at a flow rate of about 0.2 to about 1 times relative to the aqueous solution through the 1st tube, the lipids comprise a cationic lipid; and mixing the ethanol solution with the aqueous solution; the first ID and second ID and flow rates through the 1st tube and 2nd tube are selected to produce a shear force sufficiently low to preserve the integrity of the RNA; the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about ethanol, the lipid-encapsulated RNA nanoparticles having a bilayer structure.Type: GrantFiled: September 13, 2021Date of Patent: March 26, 2024Assignee: Arcturus Therapeutics, Inc.Inventors: Yanjie Bao, Brenda Clemente, Priya Prakash Karmali
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Publication number: 20230398076Abstract: A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1st tube having an inner diameter (ID) of between about 0.1? and 0.132?; b) flowing an ethanol solution comprising lipids through a 2nd tube having an ID of between about 0.005? and 0.02? at one third the flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid; and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2nd tube perpendicularly joined to the 1st tube; wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.Type: ApplicationFiled: August 28, 2023Publication date: December 14, 2023Inventors: Priya Karmali, Padmanabh Chivukula, Joseph E. Payne, Yanjie Bao, Michael Figa, Scott A. Roberts, Andreas Wagner
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Patent number: 11759515Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: GrantFiled: March 9, 2021Date of Patent: September 19, 2023Assignee: ARCTURUS THERAPEUTICS, INC.Inventors: Sean Michael Sullivan, Daiki Matsuda, Kiyoshi Tachikawa, Padmanabh Chivukula, Priya Prakash Karmali, Jared Henry Davis, Yanjie Bao
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Patent number: 11744887Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: GrantFiled: March 9, 2021Date of Patent: September 5, 2023Assignee: ARCTURUS THERAPEUTICS, INC.Inventors: Sean Michael Sullivan, Daiki Matsuda, Kiyoshi Tachikawa, Padmanabh Chivukula, Priya Prakash Karmali, Jared Henry Davis, Yanjie Bao, Amit Sagi
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Patent number: 11737979Abstract: A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1st tube having an inner diameter (ID) of between about 0.1? and 0.132?; b) flowing an ethanol solution comprising lipids through a 2nd tube having an ID of between about 0.005? and 0.02? at one third the flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid; and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2nd tube perpendicularly joined to the 1st tube; wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.Type: GrantFiled: March 18, 2020Date of Patent: August 29, 2023Assignee: Arcturus Therapeutics, Inc.Inventors: Priya Karmali, Padmanabh Chivukula, Joseph E. Payne, Yanjie Bao
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Publication number: 20230219996Abstract: Provided herein are RNA molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include RNA molecules encoding viral replication proteins and antigenic proteins or fragments thereof, and lipids. RNA molecules and compositions including them are useful for inducing immune responses.Type: ApplicationFiled: July 29, 2022Publication date: July 13, 2023Inventors: Daiki MATSUDA, Sean Michael Sullivan, Kiyoshi Tachikawa, Padmanabh Chivukula, Priya Prakash Karmali, Yanjie Bao, Amit Sagi, Rajesh Mukthavaram
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Publication number: 20230159449Abstract: Lipid formulations that encapsulate messenger RNA (mRNA) are provided herein. The mRNA can be used to express CFTR protein in vitro or in vivo. The lipid formulations can be administered via inhalation to treat cystic fibrosis.Type: ApplicationFiled: November 3, 2022Publication date: May 25, 2023Inventors: Carlos G. PEREZ-GARCIA, Kiyoshi TACHIKAWA, Daiki MATSUDA, Padmanabh CHIVUKULA, Priya Prakash KARMALI, Yanjie BAO, Jerel Boyd Lee VEGA, Rajesh MUKTHAVARAM, Amit SAGI, Yihua PEI
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Publication number: 20230149310Abstract: Nucleic acid immunization is achieved by delivering a nucleic acid (NA), e.g., a mRNA or a DNA, encapsulated within a lipid-NA nanoparticle. The NA encodes an immunogenic compound of interest. The lipid-NA nanoparticle is effective for in vivo delivery of NA to a vertebrate cell, including upon administration to a subject. The lipid-NA nanoparticle are incorporated in pharmaceutical compositions for immunizing subjects against various diseases.Type: ApplicationFiled: April 1, 2019Publication date: May 18, 2023Inventors: Padmanabh CHIVUKULA, Priya KARMALI, Christine ESAU, Jerel VEGA, Yanjie BAO, Rajesh MUKTHAVARAM, Samantha MURPHY
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Publication number: 20220168234Abstract: A method of producing lipid-encapsulated RNA nanoparticles includes flowing an aqueous solution comprising an RNA through a 1st tube having a first inner diameter (ID); the RNA comprises from about 6,000 to about 13,000 nucleotides; flowing an ethanol solution comprising lipids through a 2nd tube having a second inner diameter (ID), at a flow rate of about 0.2 to about 1 times relative to the aqueous solution through the 1st tube, the lipids comprise a cationic lipid; and mixing the ethanol solution with the aqueous solution; the first ID and second ID and flow rates through the 1st tube and 2nd tube are selected to produce a shear force sufficiently low to preserve the integrity of the RNA; the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about ethanol, the lipid-encapsulated RNA nanoparticles having a bilayer structure.Type: ApplicationFiled: September 13, 2021Publication date: June 2, 2022Inventors: Yanjie BAO, Brenda CLEMENTE, Priya Prakash KARMALI
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Publication number: 20220047519Abstract: Methods of preparing lyophilized lipid nanoparticle-nucleic acid compositions are provided. The methods comprise preparing a suspension of lipid nanoparticles with a monosaccharide and one or more excipients selected from thiosulfate, potassium sorbate, sodium benzoate, and iodixanol. Lyophilized lipid nanoparticle-nucleic acid compositions and methods of reconstituting and administering the same are further provided.Type: ApplicationFiled: August 13, 2021Publication date: February 17, 2022Inventors: Amit SAGI, Yanjie BAO, Priya Prakash KARMALI
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Publication number: 20220023442Abstract: Nucleotides encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein are provided herein. Also describe are mRNA constructs that can be used to express CFTR protein in vitro or in vivo. The mRNA constructs can be formulated in a lipid formulation and administered via inhalation to treat cystic fibrosis.Type: ApplicationFiled: April 30, 2021Publication date: January 27, 2022Inventors: Carlos G. Perez-Garcia, Kiyoshi Tachikawa, Daiki Matsuda, Padmanabh Chivukula, Priya Prakash Karmali, Yanjie Bao, Jerel Boyd Lee Vega, Rajesh Mukthavaram, Amit Sagi
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Publication number: 20210290752Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: ApplicationFiled: March 9, 2021Publication date: September 23, 2021Inventors: Sean Michael SULLIVAN, Daiki MATSUDA, Kiyoshi TACHIKAWA, Padmanabh CHIVUKULA, Priya Prakash KARMALI, Jared Henry DAVIS, Yanjie BAO
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Publication number: 20210290756Abstract: Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.Type: ApplicationFiled: March 9, 2021Publication date: September 23, 2021Inventors: Sean Michael SULLIVAN, Daiki MATSUDA, Kiyoshi TACHIKAWA, Padmanabh CHIVUKULA, Priya Prakash KARMALI, Jared Henry DAVIS, Yanjie BAO, Amit SAGI
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Publication number: 20210284974Abstract: The present disclosure describes compositions and methods for treating ornithine transcarbamylase (OTC) deficiency. The compositions include a lipid formulation and messenger RNA (mRNA) encoding an OTC enzyme. The lipid formulations can comprise an ionizable cationic lipid in a lipid nanoparticle encapsulating the mRNA.Type: ApplicationFiled: March 3, 2021Publication date: September 16, 2021Inventors: Padmanabh Chivukula, Priya Prakash Karmali, Kiyoshi Tachikawa, Suezanne E. Parker, Marciano Rodriguez Sablad, Pattraranee Limphong, Yanjie Bao, Jerel Boyd Lee Vega
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Publication number: 20200297634Abstract: A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1st tube having an inner diameter (ID) of between about 0.1? and 0.132?; b) flowing an ethanol solution comprising lipids through a 2nd tube having an ID of between about 0.005? and 0.02? at one third the flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid; and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2nd tube perpendicularly joined to the 1st tube; wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.Type: ApplicationFiled: March 18, 2020Publication date: September 24, 2020Inventors: Priya KARMALI, Padmanabh Chivukula, Joseph E. Payne, Yanjie Bao
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Publication number: 20120262067Abstract: A battery operated device such as a road stud comprises a micro-controller, a battery, and a light source. The micro-controller is configured to operate the battery to provide a train of power pulses to the light source, and each power pulse has a characteristic pulse period and a characteristic duty cycle comprising an on-cycle and an off-cycle. The micro-controller is turned on during the on-cycle and turned into a power saving or sleep mode during the off cycle. Setting the micro-controller into the power saving or sleep mode during the off cycle of the power pulse means substantial battery power saved to extend the operation time per charging of the battery.Type: ApplicationFiled: January 5, 2011Publication date: October 18, 2012Applicant: ECOTECH ENVIRONMENTAL TECHNOLOGY LTD.Inventors: Siu Chung Tam, Ka Wai Eric Cheng, Yanjie Bao, Daohong Wang, Wai Pang Yau