Abstract: A method for preparing a water-soluble rutin powder includes: heating and mixing a plant polysaccharide substance, a filler, and water to obtain a wall material solution; adding an antioxidant and a rutin powder into the wall material solution at a first temperature, followed by emulsification and dispersion, pH adjustment, and high-pressure homogenization to obtain a rutin powder emulsion; and carrying out heating fusion of the rutin powder emulsion to obtain a water-soluble rutin powder. With the preparation method, a water-soluble rutin product is obtained through emulsification, high-pressure homogenization, ultra-high-temperature fusion, drying and other processes by using modified starch and a plant polysaccharide as a wall material and rutin as a core material.

Abstract: The present invention pertains to the field of natural fermentation pigments and specifically relates to a method for preparing red Monascus pigment and its applications. The method disclosed herein enables an extraction yield of red Monascus pigment of more than 85%. Compared to red Monascus pigments obtained by conventional extraction techniques, the red Monascus pigment prepared by the present method exhibits significantly improved extraction yield, stability, and antioxidant capacity. In particular, the pigment possesses a deep red hue, making it especially suitable for use in baked goods. The improved properties of the pigment facilitate its broader applicability and commercial potential.

Abstract: A method for preparing high-purity gardenia yellow pigment includes: taking gardenia fruit, performing degreasing and impurity removal treatment on gardenia dry powder, and preparing a crude extract of gardenia dry powder by supercritical fluid extraction combined with a pre-formulated pigment carrier. A pigment adsorption resin is then prepared, followed by wet column packing of the pigment adsorption resin. The crude pigment extract is loaded onto the column for adsorption, and the pigment is eluted using a 45% ethanol solution, separating the yellow pigment solution and impurity solution. By employing the degreasing and impurity removal treatment of gardenia dry powder, and using the pigment adsorption resin to purify the crude pigment extract, the invention achieves a high-purity and high-color-value gardenia yellow pigment, while also enabling the separation of gardenia glycoside and chlorogenic acid from the gardenia yellow pigment.

Abstract: The present invention discloses a microencapsulated gardenia yellow pigment and its preparation method, relating to gardenia yellow pigment microencapsulation technology. The present invention microencapsulates gardenia yellow pigment using a co-encapsulation polymer as the microcapsule wall material. Gardenia yellow pigment exhibits good light and heat resistance under neutral or slightly alkaline conditions; however, its light and heat resistance decreases significantly and it is prone to browning under low pH conditions. To avoid excessive release under acidic conditions, the invention employs a co-encapsulation polymer to construct a pH-responsive microcapsule, which slows down or halts the release rate in acidic conditions, while accelerating the release in alkaline conditions. In this application, polyvinylpyridine is used as a release switch.

Abstract: A method of preparing a flavor composition containing D-allulose includes: (1) weighing D-allulose, mogroside, steviol glycoside and trichlorosucrose in parts by weight, and mixing them evenly to obtain a mixed powder; (2) degassing, compressing, and rolling the mixed powder, and extruding the raw materials into flakes; and (3) crushing and granulating the shaped flakes to obtain the flavor composition. D-allulose is used as the main raw material, and added high-intensity sweeteners as auxiliary raw materials. After the raw materials are mixed evenly, dry granulation is adopted, and the powder is degassed and pre-compressed by using the crystal water in the raw materials, and squeezed by a hydraulic roller to form a bonding force between the various sweetener molecules to form flakes, and the flakes are processed by processes such as crushing and granulating to obtain the flavor composition granules containing D-allulose with the various sweeteners integrated uniformly as a whole.

Abstract: The present invention belongs to the field of natural pigment modification and specifically relates to an oil-soluble beetroot red pigment, its preparation method and application. An aqueous solution containing beetroot red pigment and phospholipids is added to an aqueous solution containing water-soluble proteins and/or water-soluble peptides, and the mixture is dried to obtain an oil-soluble beetroot red pigment composite powder. The powder is subjected to low-temperature ultra-fine grinding to produce ultra-fine oil-soluble beetroot red pigment powder. Finally, the ultra-fine powder is added to vegetable oil and processed through a ball mill-colloid mill cycle to obtain the oil-soluble beetroot red pigment. The oil-soluble beetroot red pigment requires no chemical modification and contains no synthetic emulsifiers or additives.

Abstract: The present invention relates to an enzyme combination, genetically engineered bacteria, and application thereof in producing D-psicose. Glucose isomerase and D-psicose 3-epimerase from specific sources are separately co-expressed in Bacillus subtilis, a combination enzyme containing glucose isomerase and D-psicose 3-epimerase is obtained through fermentation, and the combination enzyme is used to catalyze a glucose substrate to perform isomerization to eventually prepare D-psicose. The method provided in the present invention can clearly increase a conversion rate of D-psicose, which greatly reduces the time of converting glucose into D-psicose. In addition, inexpensive glucose is used as a raw material, so that the production costs of D-psicose are greatly reduced. In the present invention, fructose syrup may be synchronously produced, so that the input for a whole set of production lines of fructose syrup is omitted, thereby greatly improving the economic benefits.

Abstract: The present invention relates to an enzyme combination, genetically engineered bacteria, and application thereof in producing D-psicose. In the present invention, glucose isomerase and D-psicose 3-epimerase from specific sources are co-expressed in Bacillus subtilis. A crude enzyme preparation is obtained by fermenting obtained engineered bacteria, and isomerization is performed by using high-concentration glucose as a substrate. Fructose syrup and D-psicose syrup are obtained by performing isolation, purification, and concentration. The enzyme combination provided in the present invention has a high catalytic rate for a substrate, and can clearly increase a conversion rate of D-psicose. In addition, inexpensive glucose is used as a raw material, so that the production costs of D-psicose are greatly reduced.

Abstract: The invention pertains to the technical field of natural product microencapsulation, specifically focusing on a water-soluble ginger oleoresin microcapsule, along with its preparation method and applications. This invention utilizes modified starch, plant polysaccharides, and similar materials as wall materials, with ginger oleoresin serving as the core material. The ginger oleoresin microcapsules are produced through processes including molecular coating, emulsification, high-pressure homogenization, constant temperature standing, and drying. The resulting product is characterized by a high gingerol content, excellent water solubility, low spiciness in solution, and strong stability in acidic environments. It fulfills the requirements of high stability, no precipitation, low spiciness, and a pleasant thermal sensation in low pH environments, making it suitable for use in food, beverages, health foods, medicines, cosmetics, and other applications.

Abstract: A method for extracting oils from sauces while simultaneously determining fat content, peroxide value, and acid value is disclosed. The method is simple to operate and requires uncomplicated equipment. It features a short testing cycle and high efficiency, and suitable for the extraction of oils from oil-rich emulsified encapsulated sauces (such as salad dressings) with high extraction rates. The determination of peroxide value and acid value is not affected by pretreatment methods, ensuring more representative results. It addresses the challenges of difficult oil separation and extraction from oil-rich emulsified encapsulated sauces and solves significant issues encountered when using acid hydrolysis and alkaline hydrolysis methods to determine peroxide value and acid value. It enables the simultaneous monitoring of three physicochemical indicators (fat content, peroxide value, and acid value).

Abstract: The invention discloses a gardenia blue pigment with high brightness and low redness and a preparation method therefor, and belongs to the technical field of pigment extraction. Genipin, an enzymolysis product of gardenia extract, is used as a raw material. Genipin and amino acids (phenylalanine, tryptophan, glycine, or tyrosine) are weighed at a molar ratio of 1:(0.375-0.75), and subjected to a polymerization reaction under a conventional condition to generate a gardenia blue precursor. The gardenia blue precursor is oxidized to develop a color, refined by using ultrafiltration membrane, and finally spray-dried to obtain the gardenia blue pigment with high brightness, low redness, and low cost.

Abstract: A method for extracting oils from sauces while simultaneously determining fat content, peroxide value, and acid value is disclosed. The method is simple to operate and requires uncomplicated equipment. It features a short testing cycle and high efficiency, and suitable for the extraction of oils from oil-rich emulsified encapsulated sauces (such as salad dressings) with high extraction rates. The determination of peroxide value and acid value is not affected by pretreatment methods, ensuring more representative results. It addresses the challenges of difficult oil separation and extraction from oil-rich emulsified encapsulated sauces and solves significant issues encountered when using acid hydrolysis and alkaline hydrolysis methods to determine peroxide value and acid value. It enables the simultaneous monitoring of three physicochemical indicators (fat content, peroxide value, and acid value).

Abstract: A method for preparing beta-carotene from pepper pigment includes: (1) enzymatic hydrolysis of paprika oleoresin to obtain a paprika red pigment hydrolysate; (2) extraction of the paprika red pigment hydrolysate to obtain a paprika red pigment solution; (3) refined filtration to obtain a paprika red pigment filtrate; (4) adsorption: carrying out chromatographic separation with a medium-pressure silica gel chromatographic column, and collecting an effluent from the chromatographic column; (5) elution of beta-carotene; (6) elution of yellow pigments; (7) elution of red pigments; and (8) elution of orange pigments. The invention combines enzymatic hydrolysis with medium-pressure column chromatography for the first time, where chromatographic silica gel with smaller particle size is used for adsorption, and four-stage elution treatment is carried out, so that beta-carotene with a content of more than 20% can be directly separated, and at the same time, reddish paprika red pigment with higher quality is prepared.

Abstract: A method for preserving a fermentation broth of an engineered strain of D-psicose-3-epimerase includes: adding a lysozyme into a fermentation broth of an engineered strain of D-psicose-3-epimerase, stirring to make the OD600 of the fermentation broth less than or equal to 1 to obtain an enzyme solution; and adjusting the enzyme solution to be weakly alkaline with an alkali liquor, and storing the weakly alkaline enzyme solution at room temperature. According to the invention, the enzyme solution is obtained by adding the lysozyme after fermentation, and then adjusted to be weakly alkaline. The lysozyme can dissolve the engineered strain to release the enzyme, and can also inhibit the growth of microorganisms. The alkaline environment of the enzyme solution can further inhibit the growth of other microorganisms in the enzyme solution. Therefore, the storage life of the enzyme solution is prolonged while ensuring the enzyme activity.

Abstract: Please replace the following substitute abstract for the abstract currently on file: A method for preparing a water-soluble rutin powder includes: heating and mixing a plant polysaccharide substance, a filler, and water to obtain a wall material solution; adding an antioxidant and a rutin powder into the wall material solution at a first temperature, followed by emulsification and dispersion, pH adjustment, and high-pressure homogenization to obtain a rutin powder emulsion; and carrying out heating fusion of the rutin powder emulsion to obtain a water-soluble rutin powder. With the preparation method, a water-soluble rutin product is obtained through emulsification, high-pressure homogenization, ultra-high-temperature fusion, drying and other processes by using modified starch and a plant polysaccharide as a wall material and rutin as a core material.

Abstract: The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

Abstract: The invention provides a D-psicose crystal and a preparation method thereof. The method comprises: adding a seed crystal to a D-psicose solution and stirring uniformly, subjecting the solution to crystallization by gradient cooling and constant-temperature crystallization alternately at 50-30° C., stopping the crystallization until the temperature of the solution is 30° C., separating the crystal by centrifugation, washing and drying, to obtain a D-psicose crystal. Rather than evaporative crystallization, organic solvent-assisted crystallization and ultrasonication, controllable crystallization by gradient cooling, constant-temperature crystallization, and other technical means are employed. Thus, the invention has low requirements for equipment, simple process, low energy consumption, and low preparation cost, and thus particularly suitable for large-scale industrial production and processing. The purity of the D-psicose crystal obtained is ?98.

Abstract: A method of preparing paprika red pigment with low polycyclic aromatic hydrocarbons includes (1) drying, crushing and granulating mature peppers to obtain pepper particles; (2) extracting the pepper particles with a vegetable oil in a rotocel extractor to obtain a paprika red pigment solution; (3) extracting the paprika red pigment solution with an ethanol solution in a liquid-liquid extractor to remove spicy substances; (4) running the paprika red pigment solution through a low-pressure chromatography column to remove polycyclic aromatic hydrocarbons; and (5) concentrating the paprika red pigment solution to obtain the paprika red pigment with low polycyclic aromatic hydrocarbons.

Abstract: A method of preparing a flavor composition containing D-allulose includes: (1) weighing D-allulose, mogroside, steviol glycoside and trichlorosucrose in parts by weight, and mixing them evenly to obtain a mixed powder; (2) degassing, compressing, and rolling the mixed powder, and extruding the raw materials into flakes; and (3) crushing and granulating the shaped flakes to obtain the flavor composition. D-allulose is used as the main raw material, and added high-intensity sweeteners as auxiliary raw materials. After the raw materials are mixed evenly, dry granulation is adopted, and the powder is degassed and pre-compressed by using the crystal water in the raw materials, and squeezed by a hydraulic roller to form a bonding force between the various sweetener molecules to form flakes, and the flakes are processed by processes such as crushing and granulating to obtain the flavor composition granules containing D-allulose with the various sweeteners integrated uniformly as a whole.

Abstract: The invention discloses a gardenia blue pigment with high brightness and low redness and a preparation method therefor, and belongs to the technical field of pigment extraction. Genipin, an enzymolysis product of gardenia extract, is used as a raw material. Genipin and amino acids (phenylalanine, tryptophan, glycine, or tyrosine) are weighed at a molar ratio of 1:(0.375-0.75), and subjected to a polymerization reaction under a conventional condition to generate a gardenia blue precursor. The gardenia blue precursor is oxidized to develop a color, refined by using ultrafiltration membrane, and finally spray-dried to obtain the gardenia blue pigment with high brightness, low redness, and low cost.