Abstract: The present application belongs to the technical field of immunotherapy, and specifically relates to a fusion protein for reversing a tumor microenvironment, a tumor immunosuppressive and resistant CAR and an expression vector, an immune cell and the use. The fusion protein is combined with different CAR structures of different targets, such as CEA, CD19, PSCA and BCMA, to form the tumor immunosuppressive and resistant CAR, or is used in combination with same to target and kill CD47 positive tumor cells; and the tumor immunosuppressive and resistant CAR and immune cells break through the influences of inhibitory signals on the function of CAR-T, which realizes the effectiveness of CAR-T treatment and can also ensure a certain degree of safety at the same time.

Abstract: A method for calculating an anchoring area of a ship includes steps of obtaining ship trajectories of ships within a certain time period in an anchorage; screening out ship trajectories including an anchoring process and eliminating trajectory points in a non-anchored state in the ship trajectories to obtain anchoring trajectories of anchored ships; clustering anchoring points in each of the anchoring trajectories, using a cluster center as an anchoring position point of each of the anchored ships; establishing an anchoring data set according to the anchoring position points; selecting anchoring data records in a predetermined time period in the anchoring data set; establishing an anchored ship position point set corresponding to the predetermined time period; and establishing Thiessen polygons corresponding to the anchoring position points; calculating an area of each of the Thiessen polygons to obtain an anchoring area of a corresponding anchored ship.

Abstract: A method and a system for determining a safe under keel clearance of an ultra-large ship are provided. The method comprises: acquiring operation parameter values of the ship; obtaining fluid pressure according to the values; obtaining a squat force and a trim moment of the ship according to the pressure; establishing a mirror image model based on speed potential to establish a squat clearance calculation model for the ship; determining a half-wave rising height with above calculation model; obtaining draught and trim changes according to the squat force and the trim moment, to determine a maximum squat clearance of the hull; determining the safe under keel clearance; and controlling the squat clearance of the ship according to the safe under keel clearance of the ship, to avoid navigation dangers, and improve the loading rate.

Abstract: A method and a system for determining a safe under keel clearance of an ultra-large ship are provided. The method comprises: acquiring operation parameter values of the ship; obtaining fluid pressure according to the values; obtaining a squat force and a trim moment of the ship according to the pressure; establishing a mirror image model based on speed potential to establish a squat clearance calculation model for the ship; determining a half-wave rising height with above calculation model; obtaining draught and trim changes according to the squat force and the trim moment, to determine a maximum squat clearance of the hull; determining the safe under keel clearance; and controlling the squat clearance of the ship according to the safe under keel clearance of the ship, to avoid navigation dangers, and improve the loading rate.

Abstract: Provided in the present invention are an improved hinge area and the use of same in constructing CAR skeleton. The amino acid sequence of the improved hinge area is as shown in SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.3, and the hinge area can prolong the survival of CAR-T cells in vivo and/or improve the capability of CAR-T cells to infiltrate tumors.

Abstract: A method and a system are disclosed for processing online joint guarantee. After a server receives an application from a user, the server provides various types of joint guarantee grouping to the user for selection. The server creates a joint guarantee group having a unique ID according to the selection of the user, organizes the information of the joint guarantee group into a data packet and sends the data packet to a bank system to be approved. The method potentially improves the success rate of joint guarantee grouping and removes the geographical limitations. Through the Internet, the method makes it possible to join companies of different geographical locations for online joint guarantee. The method also establishes a complete online alert mechanism, and realizes computerized loan risk control to improve the business credit system.