Abstract: A tissue engineering material for nerve injury repair, a preparation method therefor and an application thereof. The tissue engineering material for nerve injury repair is an N-cadherin crosslinked linear ordered collagen scaffold. By crosslinking N-cadherin with a linear ordered collagen scaffold, the prepared tissue engineering material can efficiently induce migration of neural stem cells towards an injury region so that the neural stem cells are enriched in the injury region, and can effectively inhibit deposition of inhibitory factors such as chondroitin sulfate proteoglycan, promote differentiation of the neural stem cells into neurons, and then promote recovery of electrophysiological and motion functions. The N-cadherin crosslinked linear ordered collagen scaffold also has a stable ordered topological structure and excellent mechanical properties, and can be used to repair nerve injuries such as spinal cord injury.

Abstract: Provided is a method including hybridizing a polynucleotide to a template, contacting a first template nucleotide 5-prime adjacent to a 5-prime-most nucleotide of the plurality of nucleotides that are complementary to the polynucleotide with a polymerase and a charge-tagged nucleotide, wherein the charge-tagged nucleotide is complementary to the first template nucleotide and includes a charge tag attached to a 5-prime polyphosphate of the charge-tagged nucleotide.

Abstract: The present invention provides methods for remodeling gut microbiome to a desired state. The invention also provides in vitro screening platform for identifying novel agents that can remodel dysfunctional gut microbiome.

Abstract: Provided is a device, including a conductive channel and a number of polymerase molecules bound thereto, wherein the number is between one and five and the conductive channel is to detect incorporation of a nucleotide comprising a charge tag into a nascent polynucleotide by the polymerase, and each of the one or more polymerase molecules includes a hexahistidine tag, the conductive channel includes a multivalent nickel-nitrolotriacetic acid complex, and the hexahistidine tag is bound to the multivalent nickel-nitrolotriacetic acid complex. Also provided are a method of attaching a polymerase to a conductive channel of a device and a method of using the device.

Abstract: The present invention provides cyclic compounds that are useful in preventing or treating atherosclerosis or related disorders. The invention also provides therapeutic methods for treating or preventing various diseases or disorders associated with or mediated by atherosclerosis. Further provided in the invention are methods of screening for anti-atherosclerotic cyclic peptides with improved properties.

Abstract: Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.

Abstract: The present invention provides cyclic compounds that are useful in preventing or treating atherosclerosis or related disorders. The invention also provides therapeutic methods for treating or preventing various diseases or disorders associated with or mediated by atherosclerosis. Further provided in the invention are methods of screening for anti-atherosclerotic cyclic peptides with improved properties.

Abstract: Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.