Abstract: The invention relates to a system for platelet release from a fluid comprising in particular megakaryocytic cells comprising cytoplasmic extensions. The system allows to reproduce the pipetting process performed manually by means of a pipette, thereby permitting a continuous and automatic release of platelets. This is performed on a large scale since the geometry of the system according to the invention allows to treat large volumes of fluid with a high flow rate (of the order of a plurality of liters per hour), thereby making it particularly suitable for use on an industrial scale. The invention also relates to a method for platelet release from a fluid comprising in particular megakaryocytic cells comprising cytoplasmic extensions, said method being implemented by means of the abovementioned system.

Abstract: The invention relates to a bioreactor (1) for cell culture on a three-dimensional substrate, comprising a culture chamber (2), the inner walls of which form a vertical duct, preferably, tapered, with a diameter that widens regularly form the duct inlet to the duct outlet, means (3, 4) enabling the culture medium to flow in said vertical duct. The invention also relates to the advantageous use of these bioreactors in tissue engineering, for the production of tissue grafts, notably a bone or cartilage graft.