Abstract: The invention disclosed a method for conducting site-directed modification to a plant genome using non-inheritable materials. The method provided in the present invention specifically comprises the following steps: introducing a non-inheritable material into a cell or a tissue or a part of the plant of interest; wherein said non-inheritable material is a nuclease specific to said target fragment or an mRNA expressing said nuclease, thereby the target fragment is cleaved by said nuclease and site-directed modification to the target fragment is achieved through DNA repairing in the plant. By introducing a non-inheritable material of sequence-specific nuclease, site-directed mutation in a plant gene can be achieved, and no exogenous gene or nucleic acid fragments will be integrated into the plant as obtained. Therefore, the present invention can lead to more precise genome function study and higher biosafety in breeding.

Abstract: Provided is a method for conducting site-specific modification in a plant through gene transient expression, comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest, wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease, thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.

Abstract: The present invention provides a mutant wheat plant resistant to powdery mildew and producing method thereof, wherein the mutant wheat plant comprises a loss of function mutation in a TaMLO-A1, TaMLO-B1 and TaMLO-D1 nucleic acid sequence. The present invention also provides a method for determining the presence or absence of a mutant TaMLO-A1, TaMLO-B1 and TaMLO-D1 nucleic acid or polypeptide in a wheat plant.