Abstract: The invention discloses a low-energy consumption solvent dilution device for pre-treating samples comprising an accommodating unit, the accommodating unit including a case and an accommodating portion arranged inside the case; an input unit, which including an inlet, a second outlet and a third outlet extending into the case; a cleaning unit, which including a receiving assembly arranged below the second outlet and the third outlet, and a conveying assembly communicated with a receiving assembly. The input unit including a first tube connecting with the inlet, and a second tube and a third tube extending out of the second outlet and the third outlet, respectively. The present invention completes dispensing and diluting the sample fixative in the box, the dispersion of the fixative is reduced. Avoid unnecessary contact for operators. It improves the safety and accuracy of the experiment, and significantly reduces energy consumption and labor costs.

Abstract: A method for controlling a histotripsy using a confocal fundamental and harmonic superposition combined with hundred-microsecond ultrasound pulses, including: 1) positioning a target tissue by a monitoring and guiding system and adjusting a position of the target tissue to a focal point of a transducer; 2) first stage: controlling the confocal fundamental and harmonic superposition combined with hundred-microsecond ultrasound pulses to form a shock wave in a focal zone; wherein a negative acoustic pressure exceeds a cavitation threshold; an inertial cavitation occurs to generate boiling bubbles; the boiling bubbles collapse and achieve partial homogenization of the target tissue; 3) second stage: controlling the confocal fundamental and harmonic superposition combined with hundred-microsecond pulsed-ultrasound sequences to simultaneously irradiate a target zone and further mechanically disintegrate and homogenize the target tissue.

Abstract: A method for controlling a histotripsy using a confocal fundamental and harmonic superposition combined with hundred-microsecond ultrasound pulses, including: 1) positioning a target tissue by a monitoring and guiding system and adjusting a position of the target tissue to a focal point of a transducer; 2) first stage: controlling the confocal fundamental and harmonic superposition combined with hundred-microsecond ultrasound pulses to form a shock wave in a focal zone; wherein a negative acoustic pressure exceeds a cavitation threshold; an inertial cavitation occurs to generate boiling bubbles; the boiling bubbles collapse and achieve partial homogenization of the target tissue; 3) second stage: controlling the confocal fundamental and harmonic superposition combined with hundred-microsecond pulsed-ultrasound sequences to simultaneously irradiate a target zone and further mechanically disintegrate and homogenize the target tissue.