Abstract: The present invention provides a phosphonic acid compound and a prodrug thereof, and preparation methods for and uses of the phosphonic acid compound and the prodrug thereof. Specifically, the present invention provides a compound of formula (I) or formula (I-A), wherein the compound of formula (I-A) is a prodrug form of the compound of formula (I). Upon experimental verification, the phosphonic acid compound of the present invention has good ENPP1 kinase inhibition activity, and can be used as a therapeutic agent for ENPP1-related diseases, and the phosphonic acid compound has good metabolic stability in-vivo. In addition, the phosphonate or amide prodrug compound provided by the present invention has relatively high oral bioavailability, and can overcome the defect that a phosphonic acid original drug compound cannot be taken orally.

Abstract: This invention provides an immobilized G-protein coupled receptor (GPCR) on a support in a liquid chromatography system. The method of the invention provides means of evaluating the attachment of an agent to a GPCR comprising the steps of: (a) immobilizing a GPCR on an artificial membrane support in a column, (b) exposing the support with the GPCR to test the agent at varying concentrations in a liquid chromatography system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test material from the column. Using this method, it is possible to evaluate the interaction of the test agent with the GPCR. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

Abstract: This invention provides a immobilized receptors on supports in liquid chromatographic systems.

Abstract: This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of: (a) immobilizing receptors on artificial membrane supports in a column, (b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test materials from the column. Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

Abstract: This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of:(a) immobilizing receptors on artificial membrane supports in a column,(b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system,(c) eluting the test agent from the column, and(d) evaluating the elution profile of the test materials from the column.Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.